Syntaxin 6 (C34B2) Rabbit mAb

• 产品编号：CST-2869P      品牌：Cell Signaling Technology       原厂货号：2869P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Syntaxin 6 (C34B2) Rabbit mAb兔单抗识别内源syntaxin 6总蛋白水平。该单克隆抗体用与人类syntaxin 6蛋白中Tyr140位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。Syntaxin6是一个广泛表达的SNARE蛋白S25C家族成员（1,2）。该蛋白包含一个氨基末端H1区域，随后是H2 SNARE区域和羧基末端膜锚定区（3）。Syntaxin6蛋白定位于反面高尔基体和内体内，通过参与多种SNARE蛋白活动调节膜转运（3-5）。依赖于细胞类型和SNARE伙伴，syntaxin6参与GLUT4转运的调节、中性粒细胞胞吐作用和颗粒分泌（6-10）。

1. Bock, J.B. et al. (2001) Nature 409, 839-841.
2. Bock, J.B. et al. (1996) J. Biol. Chem. 271, 17961-17965.
3. Wendler, F. and Tooze, S. (2001) Traffic 2, 606-611.
4. Bock, J.B. et al. (1997) Mol. Biol. Cell 8, 1261-1271.
5. Mallard, F. et al. (2002) J. Cell Biol. 156, 653-664.
6. Perera, H.K. et al. (2003) Mol. Biol. Cell 14, 2946-2958.
7. Shewan, A.M. et al. (2003) Mol. Biol. Cell 14, 973-986.
8. Martín-Martín, B. et al. (2000) Blood 96, 2574-25783.
9. Wendler, F. et al. (2001) Mol. Biol. Cell 12, 1699-1709.
10. Kuliawat, R. et al. (2004) Mol. Biol. Cell 15, 1690-1701.

原厂资料：

Specificity / Sensitivity

Syntaxin 6 (C34B2) Rabbit mAb detects endogenous levels of total syntaxin 6 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr140 of mouse syntaxin 6 protein.

Background

Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (1,2). The protein has an amino-terminal H1 domain followed by an H2 SNARE domain and a carboxy-terminal membrane anchor (3). Syntaxin 6 protein is localized to the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (3-5). Depending on cell type and SNARE parter, syntaxin 6 is involved in the regulation of GLUT4 trafficking, neutrophil exocytosis and granule secretion (6-10).

1. Bock, J.B. et al. (2001) Nature 409, 839-841.
2. Bock, J.B. et al. (1996) J. Biol. Chem. 271, 17961-17965.
3. Wendler, F. and Tooze, S. (2001) Traffic 2, 606-611.
4. Bock, J.B. et al. (1997) Mol. Biol. Cell 8, 1261-1271.
5. Mallard, F. et al. (2002) J. Cell Biol. 156, 653-664.
6. Perera, H.K. et al. (2003) Mol. Biol. Cell 14, 2946-2958.
7. Shewan, A.M. et al. (2003) Mol. Biol. Cell 14, 973-986.
8. Martín-Martín, B. et al. (2000) Blood 96, 2574-25783.
9. Wendler, F. et al. (2001) Mol. Biol. Cell 12, 1699-1709.
10. Kuliawat, R. et al. (2004) Mol. Biol. Cell 15, 1690-1701.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.