Phospho-c-Abl (Thr735) Antibody

• 产品编号：CST-2864S      品牌：CST       原厂货号：2864S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-c-Abl (Thr735) Antibody兔多抗能检测Thr735位点磷酸化的内源性 c-Abl 、Bcr-Abl 蛋白水平。这种抗体不会与其他相关蛋白发生交叉反应。该单克隆抗体通过使用与人源c-Abl蛋白Thr735位点周围残基相一致的磷酸化的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。c-Abl原癌基因编码一个非受体蛋白酪氨酸激酶，该激酶在多细胞动物中普遍表达且高度保守。c-Abl蛋白分布于胞核以及胞质中，参与调节细胞增殖、分化、凋亡细胞粘附和应激反应(1-3)。在体内，通过整合素激活、PDGF刺激以及结合到c-Jun、Nck 和RFX1 蛋白上(2,4)等各种生理刺激，能使c-Abl激酶的活力提高。调节c-Abl蛋白激酶活力的体内机制目前尚不完全清楚。Tyr245位点位于SH2和催化区之间的连接区域，Abl蛋白家族在这个位点处都是保守的。Tyr245位点的磷酸化涉及到c-Abl激酶的活化（5）。另外，位于Abl蛋白的激酶活化环处的Tyr412位点，对激酶的活性也是必需的（6）。Thr735位点位于保守的14-3-3蛋白的结合基序区，经应激反应或TPA处理后被磷酸化。Thr735位点的磷酸化在调节 c-Abl蛋白的定位以及功能上可能发挥着重要的作用。

1. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
2. Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
3. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
4. Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
5. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
6. Pluk, H. et al. (2002) Cell 108, 247-259.

原厂资料：

Specificity / Sensitivity

Phospho-c-Abl (Thr735) Antibody detects endogenous levels of c-Abl or Bcr-Abl only when phosphorylated at threonine 735. The antibody does not cross-react with other related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptide corresponding to residues surrounding Thr735 of human c-Abl. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).Thr735 is located within a conserved 14-3-3 protein binding motif region, and can be phosphorylated upon stress stimulation or TPA treatment. Phosphorylation at Thr735 may play an important role in regulating c-Abl localization as well as its function.

1. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
2. Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
3. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
4. Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
5. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
6. Pluk, H. et al. (2002) Cell 108, 247-259.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.