Plectin-1 Antibody

• 产品编号：CST-2863S      品牌：CST       原厂货号：2863S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Plectin-1 Antibody识别内源性plectin-1总蛋白。该抗体被期望与plectin-1蛋白所有亚型发生交叉反应，这是基于产生免疫原性多肽的序列。通过人工合成人源plectin-1蛋白中间序列相应的多肽片段去免疫动物从而制备出多克隆抗体。Plectin蛋白是一个广泛表达的蛋白，该蛋白与中间丝和肌动蛋白细胞骨架、机械稳定的细胞和组织有交联。Plectin蛋白也在调节肌动蛋白动力中起着一定作用，并且该蛋白用作信号分子的支架蛋白(1)。Plectin蛋白在半桥粒的稳定性有着重要作用，并且交联它们到中间丝的网架中。在plectin基因和其它编码半桥粒蛋白的基因中的突变能引起大疱性表皮松解症，该病体现在脆性皮肤和频繁的发热(1,2)。研究已经显示Plectin蛋白涉及数个信号传导通路。通过结合和隔绝RACK1激酶、activated C kinase 1 受体，它能传递信号到PKC激酶(3,4)。Plectin蛋白也涉及角蛋白架构和细胞应激反应(5)的调节、通过趋化因子受体CXCR4蛋白信号的传递(5)、AMP-activated protein kinase (AMPK)活性的调节和小鼠肌管的信号传递(6)。

1. Wiche, G. (1998) J Cell Sci 111 ( Pt 17), 2477-86.
2. Pfendner, E. et al. (2005) Exp Dermatol 14, 241-9.
3. Osmanagic-Myers, S. and Wiche, G. (2004) J Biol Chem 279, 18701-10.
4. Osmanagic-Myers, S. et al. (2006) J Cell Biol 174, 557-68.
5. Ding, Y. et al. (2008) Exp Cell Res 314, 590-602.
6. Gregor, M. et al. (2006) J Cell Sci 119, 1864-75.

原厂资料：

Specificity / Sensitivity

Plectin-1 Antibody detects endogenous levels of total plectin-1 protein. The antibody is expected to cross-react with all isoforms of plectin-1 based on the sequence of the immunogenic peptide.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human plectin-1.

Background

Plectin is a large, widely expressed protein that crosslinks the intermediate filament and actin cytoskeleton, mechanically stabilizing cells and tissues. Plectin also plays a role in the regulation of actin dynamics and acts as a scaffold for signaling molecules (1). Plectin is important in the stabilization of hemidesmosomes, crosslinking them to the intermediate filament network. Mutations in plectin and other genes coding for hemidesmosomal proteins can cause epidermolysis bullosa, a condition manifested by fragile skin and frequent blistering (1,2). Plectin has been shown too be involved in several signaling cascades. It signals to PKC through binding to and sequestering of RACK1, the receptor for activated C kinase 1 (3,4). Plectin is also involved in the regulation of cytokeratin architecture and cell stress response (4), signaling through the chemokine receptor CXCR4 (5) and regulation of AMP-activated protein kinase (AMPK) activity and signaling in mouse myotubes (6).

1. Wiche, G. (1998) J Cell Sci 111 ( Pt 17), 2477-86.
2. Pfendner, E. et al. (2005) Exp Dermatol 14, 241-9.
3. Osmanagic-Myers, S. and Wiche, G. (2004) J Biol Chem 279, 18701-10.
4. Osmanagic-Myers, S. et al. (2006) J Cell Biol 174, 557-68.
5. Ding, Y. et al. (2008) Exp Cell Res 314, 590-602.
6. Gregor, M. et al. (2006) J Cell Sci 119, 1864-75.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody