eIF4GI Antibody

• 产品编号：CST-2858S      品牌：CST       原厂货号：2858S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

eIF4GI Antibody检测内源性eIF4GI总蛋白水平。通过人工合成人源eIF4GI蛋白相应序列的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。翻译起始是一个重要的生物事件，众多因子参与了这一过程的进行。eIF4翻译起始因子家族成员结合5' m7GTP mRNA 帽子结构区域，并且解开mRNA的二级结构(1,2)。eIF4G蛋白氨基末端与eIF4E蛋白生理性作用从而刺激eIF4E蛋白结合到mRNA帽子结构(3)。 eIF4G蛋白也与eIF3 和eIF4A相互作用，同时该蛋白作为eIF4复合物的衔接分子(4)。此外，eIF4G蛋白在内部核糖体进入位点(IRES)介导的翻译起始过程起到重要作用(5,6)。eIF4G家族包含eIF4G1 (eIF4GI)、eIF4G2 (p97, DAP5 or NAT1)和eIF4G3 (eIF4GII) (7)。这些因子拥有一个同源序列，该序列能够与翻译起始因子eIF3和eIF4A相互作用。eIF4G1和eIF4G3两个因子都涉及到帽子依赖的翻译，然而，eIF4G2在细胞应激过程中IRES介导的一些基因的翻译起到重要作用(7,8)。

1. Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
2. Morley, S.J. et al. (1997) RNA 3, 1085-1104.
3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
4. De Gregorio, E. et al. (1998) RNA 4, 828-836.
5. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
6. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
7. Henis-Korenblit, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 5400-5405.
8. Nevins, T.A. et al. (2003) J. Biol. Chem. 278, 3572-3579.

原厂资料：

Specificity / Sensitivity

eIF4GI Antibody detects endogenous levels of total eIF4GI protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eIF4GI. Antibodies are purified by peptide affinity chromatography.

Background

The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).

1. Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
2. Morley, S.J. et al. (1997) RNA 3, 1085-1104.
3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
4. De Gregorio, E. et al. (1998) RNA 4, 828-836.
5. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
6. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
7. Henis-Korenblit, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 5400-5405.
8. Nevins, T.A. et al. (2003) J. Biol. Chem. 278, 3572-3579.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody