Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit mAb

• 产品编号：CST-2857S      品牌：CST       原厂货号：2857S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit mAb兔单抗能检测内源性仅丝氨酸（359位点）磷酸化的DUSP1蛋白水平。该单克隆抗体通过使用与人源DUSP1蛋白氨丝氨酸（359位点）周围残基相一致的磷酸化的合成肽段免疫动物而获得。双特异性蛋白激酶(DUSP)家族能使MAPK失活，DUSP成员的不同点在于它们底物的特异性、组织中的分布、胞外刺激的可诱导性和细胞中的定位。DUSP1/MKP1 (MAP激酶磷酸酶1）主要位于细胞核中，对p44/42 MAPK, p38 MAPK和SAPK/JNK有显著的底物特异性(1-3)。DUSP1的转录和活性受到严格的控制。首先，DUSP1通过p44/42 MAPK, p53和Jak2被转录性诱导(2,4,5)。其次，DUSP1羧端区域的丝氨酸（359、364位点）被p44/42 MAPK磷酸化，磷酸化抑制了DUSP1通过体内泛素途径的降解(6)。

1. Sun, H. et al. (1993) Cell 75, 487-93.
2. Brondello, J.M. et al. (1997) J Biol Chem 272, 1368-76.
3. Franklin, C.C. and Kraft, A.S. (1997) J Biol Chem 272, 16917-23.
4. Li, M. et al. (2003) J Biol Chem 278, 41059-68.
5. Sandberg, E.M. et al. (2004) J Biol Chem 279, 1956-67.
6. Brondello, J.M. et al. (1999) Science 286, 2514-7.

原厂资料：

Specificity / Sensitivity

Phospho-MKP1 (Ser359) (125E2) Rabbit mAb detects endogenous levels of MKP1 only when phosphorylated at serine 359.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser359 of human MKP1.

Background

MAP kinases are inactivated by a family of dual-specificity protein phosphatases (DUSP) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. MKP1 (MAP Kinase phosphatase 1) is primarily localized in the nucleus, and has broad substrate specificity towards p44/42 MAPK, p38 MAPK and SAPK/JNK (1-3). MKP1's transcription and activity are tightly regulated. First, MKP1 is transcriptionally induced through p44/42 MAPK, p53 and Jak2 (2,4,5). Second, MKP1 is phosphorylated by p44/42 MAPK at Ser359 and Ser364 in its carboxy-terminal region, which inhibits MKP1 degradation through the ubiquitin pathway (6).

1. Sun, H. et al. (1993) Cell 75, 487-93.
2. Brondello, J.M. et al. (1997) J Biol Chem 272, 1368-76.
3. Franklin, C.C. and Kraft, A.S. (1997) J Biol Chem 272, 16917-23.
4. Li, M. et al. (2003) J Biol Chem 278, 41059-68.
5. Sandberg, E.M. et al. (2004) J Biol Chem 279, 1956-67.
6. Brondello, J.M. et al. (1999) Science 286, 2514-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody