Phospho-ATR (Ser428) Antibody

• 产品编号：CST-2853P      品牌：Cell Signaling Technology       原厂货号：2853P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-ATR (Ser428) Antibody 能够检测内源性的丝氨酸(428位点)磷酸化的ATR蛋白。该多克隆抗体是由合成的人源的针对ATR蛋白近丝氨酸(428位)肽段免疫动物，利用A蛋白和多肽亲和层析纯化生产的。. 共济失调毛细血管扩张症突变蛋白激酶(ATM)和共济失调毛细血管扩张症兼Rad3相关激酶(ATR)属于PI3激酶相关激酶(PIKK)家族成员，它们能够在毗邻一个谷氨酰胺的丝氨酸或者苏氨酸残基处磷酸化多重底物，以响应DNA损伤或者复制阻遏(1-3)。尽管ATR在细胞周期信号和DNA修复过程中发挥重要作用，对于其激活却知之甚少。虽然ATR磷酸化位点一直没有公开的报道，CST已经生产抗体针对磷酸化的ATR(Ser428)并证明了该蛋白在体和紫外诱导的磷酸化。该试剂将是监测ATR激活的一个很有价值的工具。类似这种的脯氨酸导向的磷酸化位点通常是CDKs和MAPKs的靶标，并往往能显著影响蛋白质的构象(4,5)。

1. Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
2. Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.
3. Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.
4. Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66.
5. Smith, J. et al. (2010) Adv Cancer Res 108, 73-112.
6. Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.
7. Liu, S. et al. (2011) Mol Cell 43, 192-202.

原厂资料：

Specificity / Sensitivity

Phospho-ATR (Ser428) Antibody detects endogenous levels of ATR only when phosphorylated at serine 428.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser428 of human ATR. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. While there have been no published reports of phosphorylation sites on ATR, Cell Signaling Technology has produced an antibody directed against phospho-ATR (Ser428) that demonstrates in vivo and UV-induced phosphorylation of this protein. This reagent could prove to be a valuable tool for monitoring ATR activation. Proline-directed phosphorylation sites like this one are often targeted by CDKs and MAPKs and can often dramatically affect protein conformation (4,5).

1. Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
2. Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.
3. Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.
4. Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66.
5. Smith, J. et al. (2010) Adv Cancer Res 108, 73-112.
6. Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.
7. Liu, S. et al. (2011) Mol Cell 43, 192-202.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody