Vav2 (C64H2) Rabbit mAb

• 产品编号：CST-2848S      品牌：CST       原厂货号：2848S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Vav2 (C64H2) Rabbit mAb兔单抗检测内源性Vav2总蛋白。基于免疫原性多肽的序列，该抗体不被期望与Vav1或Vav3蛋白发生交叉反应。通过人工合成人源Vav2蛋白羧基端周围相应的多肽片段去免疫动物从而制备出单克隆抗体。Vav蛋白是属于Rho/Rac小GTPases的鸟苷酸交换因子（guanine nucleotide exchange factor, GEF）Dbl家族。三种已鉴定的哺乳类Vav蛋白(Vav1, Vav2 and Vav3)它们的蛋白表达是不同的。Vav1蛋白仅在造血细胞中表达，并且涉及形成免疫突触。Vav2和Vav3蛋白大量表达。Vav蛋白包含Dbl同源区域，这给予GEF活性，以及蛋白质相互作用区域，该区域允许它们调节肌动蛋白细胞骨架组织的信号通路(1)。磷酸化刺激Rho/Rac的Vav蛋白GEF活性(2,3) 。

1. Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
2. Crespo, P. et al. (1997) Nature 385, 169-172.
3. Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.

原厂资料：

Specificity / Sensitivity

Vav2 (C64H2) Rabbit mAb detects endogenous levels of total Vav2 protein. The antibody is not expected to cross-react with Vav1 or Vav3 based on the sequence of the immunogenic peptide.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Vav2.

Background

Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).

1. Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
2. Crespo, P. et al. (1997) Nature 385, 169-172.
3. Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.