Phospho-C/EBPα (Ser21) Antibody

• 产品编号：CST-2841S      品牌：CST       原厂货号：2841S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-C/EBPα(Ser21)抗体识别内源性的Ser21磷酸化的C/EBPα蛋白水平。此抗体与其它磷酸化的C/EBP亚型没有交叉反应。该多克隆抗体用与人C/EBPα中Ser21位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化制得。CCAAT/增强子结合蛋白（C/EBP）是一个转录因子家族，对细胞分化、终端功能和炎症反应十分关键（1）。目前已确定该家族的6个成员（C/EBPα, β, δ, γ, ε, ζ），它们分布在不同组织（1）。由不同的启动子开始转录，可以得到两种不同的C/EBPα（p42和p30），两者均是强转录激活因子（2）。有报道发现胰岛素和胰岛素样生长因子1刺激C/EBPα的去磷酸化，在胰岛素诱导的GLUT4转录抑制中扮演重要角色（3）。C/EBPα被GSK3在Thr222, Thr226, 和Ser230的磷酸化可能在脂肪生成中起作用（4）。

1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.
5. Radomska, H.S. et al. (2006) J Exp Med 203, 371-81.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Bovine

Specificity / Sensitivity

Phospho-C/EBPalpha (Ser21) Antibody detects endogenous levels of C/EBPalpha only when phosphorylated at serine 21. This antibody does not cross-react with other phosphorylated C/EBP isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human C/EBPalpha. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-1 stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226 and Ser230 by GSK3 seems to be required for adipogenesis (4).

1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.
5. Radomska, H.S. et al. (2006) J Exp Med 203, 371-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody