Neurofilament-M (RMO 14.9) Mouse mAb

• 产品编号：CST-2838P      品牌：Cell Signaling Technology       原厂货号：2838P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Neurofilament-M (RMO 14.9)Mouse mAb 鼠单抗识别内源性的总Neurofilament-M蛋白。该单克隆抗体用大鼠Neurofilament蛋白中链免疫动物而制成。细胞骨架由三种胞内纤维构成：微丝，中间纤维和微管。Neurofilament是神经元中发现的主要的中间纤维，是组成轻 (NFL)、中 (NFM)、重 (NFH)三种纤维的亚基。与其它中间纤维蛋白的结构类似，neurofilament也具有球状的氨基端头，中央α-螺旋杆结构域和一个羧基端尾。它的前体纤维由一种异型四聚体单位（NFL-NFM和NFL-NFH）构成，八条这样的前体纤维组成一个典型的10nm的中间纤维。neurofilament在轴突径向生长和确定轴突口径中起作用，微管参与轴突伸长。PKA磷酸化NFL和NFM的头端结构域，抑制neurofilament聚集（3,4）。Neurofilament的聚集在许多人类神经系统疾病中被发现，包括帕金森病（与Lewy小体中α-synuclein伴随），阿尔茨海默氏病，腓骨肌萎缩症，肌萎缩性侧索硬化症（ALS）（1）。

1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

原厂资料：

Specificity / Sensitivity

Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with rat neurofilament, medium chain.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody