Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb

• 产品编号：CST-2827P      品牌：Cell Signaling Technology       原厂货号：2827P
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗只能检测内源水平的ser70位点磷酸化的Bcl-2蛋白。在内源水平上，此抗体与未磷酸化的Bcl-2和Bcl-2家族的其它成员不发生交叉反应。该单克隆抗体是采用合成的人 Bcl-2 蛋白 ser70 位点周围残基相对应的磷酸化肽段来免疫动物而获得。Bcl-2 通过抑制线粒体细胞色素C的释放而对一系列的凋亡因素做出响应，从而发挥其促进细胞存活的功能(1)。这提示其在调节线粒体钙离子的动态平衡和质子流方面发挥着作用(2)。在Bcl-2中已经发现了几个磷酸化位点，包括Thr56, Ser70, Thr74 和 Ser87 (3)。这些磷酸化位点可能是ASK1/MKK7/JNK1通路的靶点,并且Bcl-2的磷酸化可能是有丝分裂活动的一个标志(4,5)。在T淋巴细胞糖（肾上腺）皮质激素诱导的凋亡过程中，Bcl-2在Thr56或者Ser87位点的突变能够抑制其抗凋亡的活性(6)。白细胞介素3 和JNK诱导的Bcl-2在Ser70 位点的磷酸化可能是其增强抗凋亡作用所必需的(7)。

1. Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.
2. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.
3. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.
4. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.
5. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.
6. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.
7. Deng, X. et al. (2001) J Biol Chem 276, 23681-8.

原厂资料：

Specificity / Sensitivity

Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb detects endogenous levels of Bcl-2 only when phosphorylated at serine 70. The antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 70 of human Bcl-2.

Background

Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

1. Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.
2. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.
3. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.
4. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.
5. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.
6. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.
7. Deng, X. et al. (2001) J Biol Chem 276, 23681-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.