MEP50 Antibody

• 产品编号：CST-2823S      品牌：CST       原厂货号：2823S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MEP50 Antibody能够检测内源性MEP50总蛋白水平。通过合成的与人源MEP50蛋白Ser225位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过多肽亲和层析纯化获得。MEP50(methylosome protein 50)是甲基转移酶复合体(methylosome)的组成成分，一个精氨酸甲基转移酶复合体能够修饰特定的精氨酸残基，并且这些残基在一些剪接小体Sm蛋白的精氨酸和甘氨酸富足区域发现。MEP50对于甲基转移酶复合体的活性是非常重要，并且可能调节Sm蛋白转换到SMN (survival of motor neurons)复合体，而SMN是U snRNPs组装的早期过程。Methylosome和SMN复合物对剪接小体snRNPs的组装有非常重要的作用（1）。MEP50是WD序列重复的蛋白，它可能对于多种蛋白在methylosome蛋白之间相互作用提供了一个界面（1）。它结合到JBP1，JBP1是一个methylosome的精氨酸蛋白甲基转移酶成分。MEP50已经被证明与CTD phosphatase FCP1 (CTDP1)以及SUZ12相互作用，CTD phosphatase FCP1 (CTDP1)可能是一个与转录的延长和剪切过程关联的蛋白（2），而SUZ12是属于Polycomb group (PcG) 蛋白且涉及翻译的抑制（3）。JBP1和MEP50已经证明也和甲基化Cp-G结合蛋白复合体MBD2/NuRD有相互作用（4）。

1. Friesen, W.J. et al. (2002) J Biol Chem 277, 8243-7.
2. Licciardo, P. et al. (2003) Nucleic Acids Res 31, 999-1005.
3. Furuno, K. et al. (2006) Biochem Biophys Res Commun 345, 1051-8.
4. Le Guezennec, X. et al. (2006) Mol Cell Biol 26, 843-51.

原厂资料：

Specificity / Sensitivity

MEP50 Antibody detects endogenous levels of total MEP50 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ser225 of human MEP50. Antibodies are purified by peptide affinity chromatography.

Background

MEP50 (methylosome protein 50) is a component of the methylosome, a protein arginine methyltransferase complex that modifies specific arginine residues found in arginine- and glycine-rich regions of some spliceosomal Sm proteins. MEP50 is important for methylosome activity and may regulate the transfer of Sm proteins to the SMN (survival of motor neurons) complex, an early step in the assembly of U snRNPs. Both the methylosome and the SMN complex are essential for the assembly of spliceosomal snRNPs (1). 
 MEP50 is a WD repeat protein that may provide an interface for multiple protein interactions between methylosome proteins. (1). It binds to JBP1, an arginine protein methyltransferase component of the methylosome. MEP50 has been shown to interact with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing (2), and with SUZ12, a polycomb group protein involved in transcriptional repression (3). JBP1 and MEP50 have also been reported to interact with the methyl-CpG binding protein complex MBD2/NuRD (4).

1. Friesen, W.J. et al. (2002) J Biol Chem 277, 8243-7.
2. Licciardo, P. et al. (2003) Nucleic Acids Res 31, 999-1005.
3. Furuno, K. et al. (2006) Biochem Biophys Res Commun 345, 1051-8.
4. Le Guezennec, X. et al. (2006) Mol Cell Biol 26, 843-51.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.