MLK3 Antibody

• 产品编号：CST-2817S      品牌：CST       原厂货号：2817S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MLK3 Antibody兔多抗能检测内源性总MLK3蛋白水平。该抗体不能与MLK1, MLK2，以及其他混合连接激酶 蛋白发生交叉反应。该多克隆抗体通过使用人源MLK3蛋白羧端周围残基的合成肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。混合系激酶3，MLK3，是一种丝氨酸/苏氨酸激酶，含有胺端SH3结构，以及后续的激酶结构域和两个亮氨酸拉链，cdc42/Rac1 (CRIB) 结合区域，羧端区域的一些其他结构/基序。CRIB通过它的串联亮氨酸拉链引起MLK3的二聚作用、细胞内磷酸化以及活化(1,2)。苏氨酸（277位点）和丝氨酸（281位点）对MLK3激酶的活化十分重要。（3）。丝氨酸（281位点）也能在体内激酶反应中被HPK磷酸化（3）。MLK3是SAPK/JNK应激反应途径中的MAPKKK，该应激反应途径能直接将SEK1/MKK4、MKK7磷酸化，但是MLK3是否涉及到p38应激途径的活化还有争议。（1，4）MLK3也是一种IκB激酶，能调节由CD3/CD28刺激的转录因子NF-κB的活化，暗示了MLK3在免疫反应和炎性反应中的作用 (5)。

1. Teramoto, H. et al. (1996) J. Biol. Chem. 271, 27225-27228.
2. Leung, I. W. et al. (1998) J. Biol. Chem. 273, 32408-32415.
3. Leung, I.W. and Lassam, N. (2001) J. Biol. Chem. 276, 1961-1967.
4. Tibbles, L. A. et al. (1996) EMBO J. 15, 7026-7035.
5. Hehner, S. P. et al. (2000) Mol. Cell. Biol. 20, 2556-2568.

原厂资料：

Specificity / Sensitivity

MLK3 Antibody detects endogenous levels of total MLK3 protein. This antibody does not cross-react with MLK1, MLK2 and other mixed lineage kinases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLK3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Mixed lineage kinase 3 (MLK3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. CRIB triggers the dimerization of MLK3 via its tandem leucine zippers, followed by the intramolecular phosphorylation and subsequent activation of MLK3 (1,2). Autophosphorylation of Thr277 and Ser281 is essential for MLK3 kinase activity (3). Ser281 is also phosphorylated by HPK in an in vitro kinase assay (3). MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation (1,4). MLK3 also functions as an IκB kinase and mediates the activation of the transcriptional factor NF-κB stimulated by CD3/CD28, suggesting a role for MLK3 in immune and inflammatory responses (5).

1. Teramoto, H. et al. (1996) J. Biol. Chem. 271, 27225-27228.
2. Leung, I. W. et al. (1998) J. Biol. Chem. 273, 32408-32415.
3. Leung, I.W. and Lassam, N. (2001) J. Biol. Chem. 276, 1961-1967.
4. Tibbles, L. A. et al. (1996) EMBO J. 15, 7026-7035.
5. Hehner, S. P. et al. (2000) Mol. Cell. Biol. 20, 2556-2568.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody