INCENP (P240) Antibody

• 产品编号：CST-2807S      品牌：CST       原厂货号：2807S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CENP (P240) Antibody能够检测内源性INCENP总蛋白。该多克隆抗体是由合成的人源的针对INCENP蛋白脯氨酸（240位）的肽段免疫动物，采用多肽亲和层析技术纯化生产的。 INCENP(内着丝粒蛋白抗原135 kDa，155 kDa)是一种染色体乘客蛋白，在有丝分裂过程中介导染色体分离的多重事件中至关重要(1)。在分裂前期，INCENP和染色质有关；而在前中期和中期，它转移到内着丝粒(1)。INCENP的减少可以引起异常的赤道板染色体排布、不完全染色体分离和适当的纺锤体形成以及胞质分裂的破坏(2)。INCENP是染色体乘客蛋白复合体的一部分，该复合体还包括Aurora B，borealin和survivin (2)。Aurora B 和 INCENP相互依赖以形成适当的定位(3)，在果蝇细胞和线虫胚胎中，缺少INCENP 或survivin,，Aurora B会导致无法组织着丝粒和中间体(4,5)。CDK1引起的INCENP第59位和388位苏氨酸磷酸化可导致INCENP与另一个重要有丝分裂调节子Plk1的联合作用(6)。INCENP与Plk1的相互作用对于招募Plk1至着丝粒以及中期到末期转换是必不可少的(6)。INCENP和异染色质蛋白1α (HP1) (7)和β-管蛋白(8)之间的相互作用亦有报道(8)。 Kif11:4203/7265

1. Carmena, M. and Earnshaw, W.C. (2006) Nat Cell Biol 8, 110-1.
2. Carmena, M. and Earnshaw, W.C. (2003) Nat Rev Mol Cell Biol 4, 842-54.
3. Kaitna, S. et al. (2000) Curr Biol 10, 1172-81.
4. Speliotes, E.K. et al. (2000) Mol Cell 6, 211-23.
5. Adams, R.R. et al. (2001) J Cell Biol 153, 865-80.
6. Goto, H. et al. (2006) Nat Cell Biol 8, 180-7.
7. Ainsztein, A.M. et al. (1998) J Cell Biol 143, 1763-74.
8. Wheatley, S.P. et al. (2001) Exp Cell Res 262, 122-7.

原厂资料：

Specificity / Sensitivity

INCENP (P240) Antibody detects endogenous levels of total INCENP protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Pro240 of human INCENP. Antibodies are purified by peptide affinity chromatography. 

Background

INCENP (inner centromere protein antigens 135 kDa, 155 kDa) is a chromosomal passenger protein crucial for multiple events that mediate chromosome separation during mitosis (1). At prophase INCENP is associated with chromatin whereas during prometaphase and metaphase it translocates to the inner centromere (1). Depletion of INCENP results in aberrant chromosome alignment at the metaphase plate, incomplete chromosome separation, and disruption of proper spindle formation and cytokinesis (2). INCENP is part of the chromosomal passenger complex that also contains Aurora B, borealin and survivin (2). Aurora B and INCENP are mutually dependent on each other for proper localization (3), and in Drosophila cells and C.elegans embryos that lack INCENP or survivin, Aurora B cannot organize the kinetochores and the midbody (4,5). Phosphorylation on INCENP by CDK1 on Thr59 and Thr388 leads to the association of INCENP with Plk1, another important regulator of mitotic entry and exit (6). Interaction of INCENP with Plk1 is necessary for recruitment of Plk1 to the kinetochores, and the metaphase to anaphase transition (6). Interactions have also been reported between INCENP and heterochromatin protein 1α (HP1) (7) and β-tubulin (8). 

1. Carmena, M. and Earnshaw, W.C. (2006) Nat Cell Biol 8, 110-1.
2. Carmena, M. and Earnshaw, W.C. (2003) Nat Rev Mol Cell Biol 4, 842-54.
3. Kaitna, S. et al. (2000) Curr Biol 10, 1172-81.
4. Speliotes, E.K. et al. (2000) Mol Cell 6, 211-23.
5. Adams, R.R. et al. (2001) J Cell Biol 153, 865-80.
6. Goto, H. et al. (2006) Nat Cell Biol 8, 180-7.
7. Ainsztein, A.M. et al. (1998) J Cell Biol 143, 1763-74.
8. Wheatley, S.P. et al. (2001) Exp Cell Res 262, 122-7.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20oC.
Do not aliquot the antibody.