Hexokinase I Antibody

• 产品编号：CST-2804S      品牌：CST       原厂货号：2804S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

己糖激酶I抗体识别内源性的己糖激酶I总蛋白水平。该多克隆抗体用与人类己糖激酶I氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化制得。己糖激酶催化糖酵解第一步中葡萄糖到6-磷酸葡萄糖的转化（1）。目前已确定哺乳动物己糖激酶的四个不同的亚型，分别为I, II, III, IV（葡萄糖激酶）。己糖激酶I、II、III与线粒体外膜相关联，在癌细胞保持高效率无氧酵解（Warburg效应）中至关重要（1），这补偿了快速生长增殖的癌细胞的能量需求（2,3）。

1. WARBURG, O. (1956) Science 123, 309-14.
2. Semenza, G.L. (2000) Crit Rev Biochem Mol Biol 35, 71-103.
3. Smith, T.A. (2000) Br J Biomed Sci 57, 170-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

Hexokinase I Antibody detects endogenous levels of total hexokinase I protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human hexokinase I. Antibodies are purified by peptide affinity chromatography.

Background

Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3).

1. WARBURG, O. (1956) Science 123, 309-14.
2. Semenza, G.L. (2000) Crit Rev Biochem Mol Biol 35, 71-103.
3. Smith, T.A. (2000) Br J Biomed Sci 57, 170-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.