SCD1 (C12H5) Rabbit mAb

• 产品编号：CST-2794S      品牌：CST       原厂货号：2794S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SCD1 (C12H5)兔单克隆抗体识别内源性的全SCD1总蛋白水平。该单克隆抗体用与小鼠SCD1蛋白中Leu35位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。硬脂酰CoA去饱和酶1（SCD1）是一个关键的脂肪合成酶，在内质网中发现，可催化棕榈酰CoA和硬脂酰CoA到棕榈油酰CoA（16:1）和油酰CoA（18:1）的转换（1-3）。 棕榈油酰和油酰是甘油三酯，膜磷脂和胆固醇酯的主要组成部分（1）。SCD1基因敲除小鼠显示出增高的胰岛素敏感性和减少的体内脂肪（1）。小鼠棕色脂肪组织中SCD1的中断会增强胰岛素信号，导致GLUT4易位到质膜增加,增强葡萄糖摄取（4）。此外，SCD1对饮食引起体重增加的发生（1）和肝脏胰岛素抵抗（5）是至关重要的。

1. Ntambi, J.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11482-114866.
2. Kato, H. et al. (2006) J. Cell. Sci. 119, 2342-2353.
3. Ozols, J. (1997) Mol. Biol. Cell 8, 2281-2290.
4. Rahman, S.M. et al. (2005) Am. J Physiol. Endocrinol. Metab. 288, E381-387.
5. Gutiérrez-Juárez, R. et al. (2006) J. Clin. Invest. 116, 1686-1695.

原厂资料：

Specificity / Sensitivity

SCD1 (C12H5) Rabbit mAb detects endogenous levels of total SCD1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu35 of mouse SCD1.

Background

Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl–CoA and stearoyl–CoA to palmitoleoyl–CoA (16:1) and oleoyl–CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5).

1. Ntambi, J.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11482-114866.
2. Kato, H. et al. (2006) J. Cell. Sci. 119, 2342-2353.
3. Ozols, J. (1997) Mol. Biol. Cell 8, 2281-2290.
4. Rahman, S.M. et al. (2005) Am. J Physiol. Endocrinol. Metab. 288, E381-387.
5. Gutiérrez-Juárez, R. et al. (2006) J. Clin. Invest. 116, 1686-1695.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody