Lck (73A5) Rabbit mAb

• 产品编号：CST-2787P      品牌：Cell Signaling Technology       原厂货号：2787P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lck (73A5) Rabbit mAb 兔单抗能够检测内源Lck蛋白总体水平。此单克隆抗体是通过合成人源对应的Lck Tyr505位点周围的肽段来免疫动物而获得。Src酪氨酸激酶蛋白家族包括Src, Lyn, Fyn, Yes, Lck, Blk 和 Hck, 其对真核细胞的生长和分化过程中起了重要的作用(1)。Src的活性是通过两个位点酪氨酸的磷酸化进行调节的, 但是具有相反地作用。在激酶活性环内的Tyr416 位点的磷酸化增加酶的活性,通过Csk磷酸化C-末端的Tyr527位点降低酶的活性(2)。p56lck (淋巴细胞激酶) 在胸腺细胞的成熟和激活以及外周血淋巴细胞的增殖过程中是一个关键的酶。p56lck的序列与 致癌基因 p60c-src的序列高度同源，正如其外显子-内含子的组织方式。p56lck并不参与到淋巴组织增生性疾病中，不管是过表达还是活性突变体。然而, 据报道在一些癌变的组织中如(结肠癌,肺癌和乳腺癌)，它的表达是异常的。有报道暗示着p56lck 可能是更倾向于转移失去粘连作用的细胞(3)。

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Rouer, E. (2004) Bull Cancer 91, 928-40.

原厂资料：

Specificity / Sensitivity

Lck (73A5) Rabbit mAb detects endogenous levels of total Lck protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr505 of human Lck.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).p56lck (lymphocyte cellular kinase) has been shown to be a pivotal enzyme to both maturation of thymocytes and activation and proliferation of peripheral lymphocytes. The p56lck sequence appeared highly homologous to that of the oncogene p60c-src as did its exon-intron organisation. p56lck does not appear involved in lymphoproliferative diseases, either by overexpression or activating mutations. Nevertheless, its aberrant expression has been reported in some carcinomas (colon, lung and mammary). It was suggested that p56lck could favor metastases by facilitating loss of cell adhesion (3).

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Rouer, E. (2004) Bull Cancer 91, 928-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.