Pyruvate Dehydrogenase Antibody

产品编号：CST-2784S 品牌：CST 原厂货号：2784S
产品分类：抗体 > 一抗 > 蛋白特异性一抗
应用分类：

包装：

运保温度:

–20°C.

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描述：

丙酮酸脱氢酶抗体识别内源性的丙酮酸脱氢酶总蛋白α1和α2亚基。该多克隆抗体用与人类丙酮酸脱氢酶α1和α2亚基氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用肽亲和层析纯化制得。该多克隆抗体用与人类丙酮酸脱氢酶α1和α2亚基氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用肽亲和层析纯化制得。

原厂资料：

Specificity / Sensitivity

Pyruvate Dehydrogenase Antibody detects endogenous levels of total α1 and α2 subunits of pyruvate dehydrogenase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequences of both α1 and α2 subunits of human pyruvate dehydrogenase. Antibodies are purified by peptide affinity chromatography.

Background

The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: α and β. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.