Phospho-PEA-15 (Ser104) Antibody

• 产品编号：CST-2776L      品牌：CST       原厂货号：2776L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-PEA-15 (Ser104) Antibody能够检测内源性的Ser104位磷酸化的PEA-15 蛋白。该多克隆抗体是采用合成的人源PEA-15蛋白Ser104周围残基想对应的磷酸化肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。PEA-15是一个15 kDa的磷酸化蛋白，在星形胶质细胞、成纤维细胞以及组织（包括肺和眼）中表达（1）。研究证明，该蛋白协助调控细胞的生长、死亡，及葡萄糖利用（2-4）。PEA-15的氨基末端DED结构域介导它与FADD或Erk的结合，并进一步调控Erk和细胞凋亡信号通路。PEA-15可以在羧基末端的Ser104和Ser116两个丝氨酸残基处被磷酸化。在这些位点上的磷酸化调节其与Erk和FADD的结合（2,3）。

1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

Phospho-PEA-15 (Ser104) Antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human PEA-15. Antibodies are purified by protein A and peptide affinity chromatography.

Background

PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose ultilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and futher regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).

1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody