Bax Antibody

• 产品编号：CST-2774S      品牌：CST       原厂货号：2774S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bax Antibody(Human-Specific) 能够检测内源的Bax 总蛋白。在生理水平下，此抗体不与该家族的其它成员交叉反应。该多克隆抗体是采用合成的人源 Bax lys58 周围残基相对应的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。Bax 是细胞内通过线粒体胁迫而诱导凋亡过程中一个重要的组分(1)。受到凋亡的刺激后，Bax 形成链式聚合物，并从细胞质转运到线粒体膜上(2)。通过与线粒体膜上的 pore 蛋白互作，Bax 增加了膜的通透性，这使得细胞色素C从线粒体释放，从而激活caspase-9并起始caspase 激活的凋亡信号通路 (3,4)。

1. Wei, M.C. et al. (2001) Science 292, 727-30.
2. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002.
3. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6.
4. Marzo, I. et al. (1998) Science 281, 2027-31.

原厂资料：

Specificity / Sensitivity

Bax Antibody (Human-Specific) detects endogenous levels of total Bax protein. Cross-reativity was not detected with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lysine 58 of human Bax. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).

1. Wei, M.C. et al. (2001) Science 292, 727-30.
2. Jürgensmeier, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 4997-5002.
3. Narita, M. et al. (1998) Proc Natl Acad Sci U S A 95, 14681-6.
4. Marzo, I. et al. (1998) Science 281, 2027-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.