Bcl-xL (54H6) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-2767S      品牌：CST       原厂货号：2767S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

单克隆抗体是采用合成人源Bcl-xL Asp61位点周围残基相对应的多肽来免疫动物而获得。在最优化条件下，此抗体以 F/P ratio 为 2-6 与 Alexa Fluor^® 488偶联。Bcl-xL (54H6) Rabbit mAb (Alexa Fluor^® 488 Conjugate) 兔单抗能够检测内源水平Bcl-xL总蛋白。此抗体不与Bcl-2 家族的其它成员交叉反应。Bcl-xL 通过两种不同的机制来阻止凋亡：通过与凋亡蛋白形成异源二聚体来阻止其凋亡作用(1,2)。并且可形成线粒体膜外微孔来帮助在胁迫环境下保持正常的膜状态(3)。Bcl-xL 在经微管破坏物如paclitaxel、vinblastine 和nocodazole处理后能够被JNK磷酸化 (4,5)。

1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.
6. Mirlashari, M.R. et al. (2012) Leuk Res 36, 499-508.

原厂资料：

Specificity / Sensitivity

Bcl-xL (54H6) Rabbit mAb (Alexa Fluor^® 488 Conjugate) detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL. The antibody was conjugated to Alexa Fluor^® 488 under optimal conditions with an F/P ratio of 2-6.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #2764 reacts with human, mouse, rat and monkey Bcl-xL protein. CST expects that Bcl-xL (54H6) Rabbit mAb (Alexa Fluor^® 488 Conjugate) will also recognize Bcl-xL in these species.

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.
6. Mirlashari, M.R. et al. (2012) Leuk Res 36, 499-508.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide, 2 mg/ml BSA. Store at 4°C. Protect from light.
Do not freeze.