Bcl-xL (54H6) Rabbit mAb

• 产品编号：CST-2764P      品牌：Cell Signaling Technology       原厂货号：2764P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bcl-xL (54H6) Rabbit mAb 兔单抗能够检测内源水平 Bcl-xL 总蛋白。此抗体不会与Bcl-2 家族的其它成员交叉反应。单克隆抗体是采用合成的人源Bcl-xL蛋白 Asp61位点周围残基相对应的动物多肽来免疫动物而获得。Bcl-xL 通过两种不同的机制来阻止凋亡：通过与凋亡蛋白形成异源二聚体来阻止其凋亡作用(1,2)。并且可形成线粒体膜外微孔来帮助在胁迫环境下保持正常的膜状态(3)。Bcl-xL 在经微管破坏物如paclitaxel、vinblastine 和nocodazole处理后能够被JNK磷酸化 (4,5)。

1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.

原厂资料：

Specificity / Sensitivity

Bcl-xL (54H6) Rabbit mAb detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL.

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-6.
2. Minn, A.J. et al. (1999) EMBO J 18, 632-43.
3. Vander Heiden, M.G. et al. (2001) J Biol Chem 276, 19414-9.
4. Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
5. Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.