Numb (C44B4) Rabbit mAb

• 产品编号：CST-2761S      品牌：CST       原厂货号：2761S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Numb (C44B4)Rabbit mAb兔单抗识别内源性的Numb 蛋白.单克隆抗体是通过合成肽段去免疫动物产生。这种合成肽段与邻近人源Numb蛋白Phe482氨基酸残基序列一致.Numb包含一个氨基末端磷酸化酪氨酸结合（PTB）结构域和C末端对α-衔接蛋白和EH（Eps15 同源物）结构域包含蛋白的内吞结合基序，显示在细胞吞噬中的作用（1,2）有四个哺乳动物Numb剪接异构体，差异表达，并有各自独特功能（3-5）。Numb通过促进Notch泛素降解对Notch信号通路起负向调控作用（6），蛋白在细胞分裂时不对称的入子细胞，产生的两个子细胞对Notch信号和细胞命运都有不同反应（7,8）。Numb可能被G蛋白偶联受体和PKC信号调控（9）。

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.

原厂资料：

Specificity / Sensitivity

Numb (C44B4) Rabbit mAb detects endogenous levels of Numb protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb protein.

Background

Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).

1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.