Oct-4 Antibody

• 产品编号：CST-2750S      品牌：CST       原厂货号：2750S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Oct-4抗体识别内源性的总Oct-4 蛋白。多克隆抗体是通过合成的肽段免疫动物产生。这种合成的肽段与邻近人源Oct-4蛋白羧基末端的氨基酸残基序列一致，抗体由肽段亲和层析技术纯化.Oct-4 (POU5F1)是一类高表达于未分化胚胎干细胞和原始胚胎生殖细胞中的转录因子（1）,一系列关键因子包括Oct-4, Nanog和 Sox2对保持多潜能性是必需的，Oct-4的下调显示能起始细胞分化（2,3）。Oct-4是一种非常有用的生殖细胞肿瘤标志物[4]。Oct-4存在两种剪接异构体Oct-4A and Oct-4B（5）。近来研究暗示Oct-4A异构体能给予并保持干细胞多潜能，而Oct-4B可能存在于某些体细胞和非多潜能性细胞中（6,7）。

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Oct-4 Antibody detects endogenous levels of total Oct-4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human Oct-4. Antibodies are purified by peptide affinity chromatography.

Background

Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody