FEN-1 Antibody

• 产品编号：CST-2746S      品牌：CST       原厂货号：2746S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

FEN-1 Antibody detects endogenous levels of total FEN-1 protein.FEN-1 Antibody能够检测内源性FEN-1总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Lys313 of human FEN-1. Antibodies are purified by peptide affinity chromatography.该多克隆抗体是由合成的人源的针对FEN-1蛋白赖氨酸（313位）的肽段免疫动物，采用多肽亲和层析技术纯化生产的。

原厂资料：

Specificity / Sensitivity

FEN-1 Antibody detects endogenous levels of total FEN-1 protein.FEN-1 Antibody能够检测内源性FEN-1总蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Lys313 of human FEN-1. Antibodies are purified by peptide affinity chromatography.该多克隆抗体是由合成的人源的针对FEN-1蛋白赖氨酸（313位）的肽段免疫动物，采用多肽亲和层析技术纯化生产的。

Background

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).Flap内切核酸酶1（FEN-1）是是一个结构特异的核酸酶，在DNA处理通路中具有多种功能（1,2）。 FEN-1的复制和DNA修复活性对真核细胞的基因组稳定性至关重要。通过与增殖细胞核抗原（PCNA）相互作用，FEN-1能够通过去除RNA-DNA引物来帮助协调冈崎片段成熟（3）。 FEN-1也为长补丁碱基切除修复中的双链DNA断裂的非同源末端连接所需（4,5）。 FEN-1的多功能活性受各种机制调控，包括蛋白质伴侣相互作用（6,7），翻译后修饰（8,9），以及响应细胞周期或DNA损伤的亚细胞定位（10）。

1. Shen, B. et al. (2005) Bioessays 27, 717-729.
2. Liu, Y. et al. (2004) Annu. Rev. Biochem. 73, 589-615.
3. Sakurai, S. et al. (2005) EMBO J. 24, 683-693.
4. Wu, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 1303-1308.
5. Gary, R. et al. (1999) J. Biol. Chem. 274, 4354-4363.
6. Brosh, R.M. et al. (2001) EMBO J. 20, 5791-5801.
7. Sharma, S. et al. (2004) J. Biol. Chem. 279, 9847-9856.
8. Henneke, G. et al. (2003) Oncogene 22, 4301-4313.
9. Hasan, S. et al. (2001) Mol. Cell 7, 1221-1231.
10. Qiu, J. et al. (2001) J. Biol. Chem. 276, 4901-4908.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.