MGMT Antibody

• 产品编号：CST-2739S      品牌：CST       原厂货号：2739S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MGMT Antibody能够检测内源性MGMT总蛋白。该多克隆抗体是由合成的人源的针对MGMT蛋白氨基末端肽段免疫动物，利用多肽亲和层析技术纯化生产的。MGMT（O-6-甲基鸟嘌呤-DNA甲基转移酶）是一种DNA修复酶，参与自杀反应，特异性的清除鸟嘌呤6位O的甲基或烷基官能团，恢复鸟嘌呤到其正常形式且不造成DNA断裂(1)。 MGMT可以保护细胞免受烷化毒素的损害，是针对烷化治疗药物抵抗的重要因子(2,3)。它在正常的人体组织中广泛表达(4)，在多种人类肿瘤中过度表达而在其它一些肿瘤中表观沉默。MGMT沉默是不良预后的相关标志，但是在烷化剂化疗过程中是一个很好的预测指标(5)。

1. Pegg, A.E. (2000) Mutat. Res. 462, 83-100.
2. Bobola, M.S. et al. (2005) Clin. Cancer Res. 11, 2747-2755.
3. Pollack, I.F. et al. (2006) J. Clin. Oncol. 24, 3431-3437.
4. Gerson, S.L. et al. (1986) Carcinogenesis 7, 745-749.
5. Soejima, H. et al. (2005) Biochem. Cell Biol. 83, 429-437.

原厂资料：

Specificity / Sensitivity

MGMT Antibody detects endogenous levels of total MGMT protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human MGMT. Antibodies are purified by peptide affinity chromatography. 

Background

MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O(6) position of guanine, restoring guanine to its normal form without causing DNA breaks (1). MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents (2,3). It is ubiquitously expressed in normal human tissues (4) and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy (5). 

1. Pegg, A.E. (2000) Mutat. Res. 462, 83-100.
2. Bobola, M.S. et al. (2005) Clin. Cancer Res. 11, 2747-2755.
3. Pollack, I.F. et al. (2006) J. Clin. Oncol. 24, 3431-3437.
4. Gerson, S.L. et al. (1986) Carcinogenesis 7, 745-749.
5. Soejima, H. et al. (2005) Biochem. Cell Biol. 83, 429-437.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody