XRCC1 Antibody

• 产品编号：CST-2735S      品牌：CST       原厂货号：2735S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

多克隆抗体是由合成的人源的针对XRCC1蛋白的精氨酸（300位）的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。X射线修复交叉互补蛋白1(XRCC1)是一个DNA修复蛋白，它在伴随离子辐射和烷化剂而至的单链断裂修复和碱基清除修复中都发挥重要作用(1)。XRCC1能够充当支架蛋白并通过与其它几个修复蛋白相互作用来协调DNA脱碱基位点修复(2)。至少八个XRCC1蛋白伴侣已经被鉴定，包括多核苷酸激酶PNK(3)，DNA连接酶III (4,5)，多(ADP-核糖)聚合酶(6)和PCNA (7)。XRCC1基因的突变和多态性可充当诊断标志物并和多种不同类型癌症的高风险相关(8)。

1. Brem, R. and Hall, J. (2005) Nucleic Acids Res. 33, 2512-2520.
2. Vidal, A.E. et al. (2001) EMBO J. 20, 6530-6539.
3. Whitehouse, C.J. et al. (2001) Cell 104, 107-117.
4. Caldecott, K.W. et al. (1994) Mol. Cell. Biol. 14, 68-76.
5. Nash, R.A. et al. (1997) Biochemistry 36, 5207-5211.
6. Masson, M. et al. (1998) Mol. Cell. Biol. 18, 3563-3571.
7. Fan, J. et al. (2004) Nucleic Acids Res. 32, 2193-2201.
8. Hu, Z. et al. (2005) Cancer Epidemiol. Biomarkers Prev. 14, 1810-1818.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

XRCC1 Antibody detects endogenous levels of total XRCC1 protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Arg300 of human XRCC1. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

The X-ray repair cross complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the XRCC1 gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8). 

1. Brem, R. and Hall, J. (2005) Nucleic Acids Res. 33, 2512-2520.
2. Vidal, A.E. et al. (2001) EMBO J. 20, 6530-6539.
3. Whitehouse, C.J. et al. (2001) Cell 104, 107-117.
4. Caldecott, K.W. et al. (1994) Mol. Cell. Biol. 14, 68-76.
5. Nash, R.A. et al. (1997) Biochemistry 36, 5207-5211.
6. Masson, M. et al. (1998) Mol. Cell. Biol. 18, 3563-3571.
7. Fan, J. et al. (2004) Nucleic Acids Res. 32, 2193-2201.
8. Hu, Z. et al. (2005) Cancer Epidemiol. Biomarkers Prev. 14, 1810-1818.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20oC.
Do not aliquot the antibody