纯化的抗体不能直接用于其它已知抗原的检测,它是从天然兔血浆中分离出来的,通过蛋白A亲和色谱法制备。正常Rabbit IgG是一种未结合的兔源多克隆抗体,在应用我们的SimpleChIP™ Enzymatic Chromatin IP Kits #9002 和 #9003中常规用来作为染色质免疫沉淀中非特异性IgG对照。正常兔源IgG是一种同型对照,是用来评估针对由于Fc受体结合或者其它蛋白与蛋白之间的相互作用引起的一抗的非特异性结合。一个同型对照抗体应该与被测抗体具有相同的免疫球蛋白类型和相同的浓度。
原厂资料:
Source / Purification
The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.
Description
Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific IgG control in chromatin immunoprecipitation using our SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003.
Background
Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
注意事项:
Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 μg/ml BSA and 50% glycerol. Store at -20°C.
Do not aliquot the antibody.