Bcl-w (31H4) Rabbit mAb

• 产品编号：CST-2724S      品牌：CST       原厂货号：2724S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bcl-w (31H4) Rabbit mAb 兔单抗能够检测内源水平的 Bcl-w 总蛋白。该单克隆抗体是采用合成Bcl-w的ala39位点周围残基相对应的肽段来免疫动物而获得。Bcl-2家族由一系列进化保守的蛋白组成，它们都包含Bcl-2同源结构域，能通过控制线粒体膜的通透性和释放细胞色素C来调节凋亡（1-3）。目前，已确定有四种BH结构域 (BH1-4)，并介导蛋白的相互作用。此家族蛋白根据功能和结构的同源性分为三组：促生存成员，包括Bcl-2、Bcl-xL、Mcl-1、A1 和 Bcl-w；促凋亡蛋白，包括Bax、Bak 和 Bok；仅含有BH3结构域的蛋白，包括Bad、Bik、Bid、Puma、Bim、Bmf、Noxa 和 Hrk。Bcl-2家族成员间促进死亡和抑制死亡成员间的相互作用构成了变阻模型，即通过促死亡蛋白和抑制死亡蛋白的比例来控制细胞的命运（4）。因此，促生存蛋白通过结合和对抗促凋亡成员来发挥作用。一般来讲，仅含有BH3结构域蛋白能结合和对抗促生存蛋白来增加凋亡（5）。因此，这个系统的冗余功能，比如这些家族成员的存活、组织特异性、转录和转录后翻译的调节，可能发挥不同的生理作用。促生存蛋白 Bcl-w 最初是利用基于PCR的策略寻找新的Bcl-2家族成员时鉴定的，并且发现它在骨髓起源细胞中有表达，在别的很多组织中也有表达 (6,7)。在 bcl-w 敲除的小鼠模型中，很多组织不会受到影响，但是雄鼠的精小管组织和spermatogenogenesis存在缺陷 (8,9)。

1. Cory, S. et al. (2003) Oncogene 22, 8590-607.
2. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7.
3. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13.
4. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32.
5. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
6. Gibson, L. et al. (1996) Oncogene 13, 665-75.
7. O'Reilly, L.A. et al. (2001) Cell Death Differ 8, 486-94.
8. Print, C.G. et al. (1998) Proc Natl Acad Sci U S A 95, 12424-31.
9. Ross, A.J. et al. (1998) Nat Genet 18, 251-6.

原厂资料：

Specificity / Sensitivity

Bcl-w (31H4) Rabbit mAb detects endogenous levels of total Bcl-w protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding alanine 39 of Bcl-w.

Background

The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4) that mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-survival members include Bcl-2, Bcl-xL, Mcl-1, A1 and Bcl-w; pro-apoptotic proteins include Bax, Bak and Bok, and "BH3 only" proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the "BH3-only members" can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity, transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles.The pro-survival protein Bcl-w was originally identified in a PCR-based strategy aimed at discovering novel Bcl-2 family members and was found to be expressed in cells of myeloid origin, as well as many other tissues (6,7). Most tissues from bcl-w knockout mice were unaffected, but male mice did show defects in seminiferous tubule organization and spermatogenogenesis (8,9).

1. Cory, S. et al. (2003) Oncogene 22, 8590-607.
2. Antonsson, B. and Martinou, J.C. (2000) Exp Cell Res 256, 50-7.
3. Sharpe, J.C. et al. (2004) Biochim Biophys Acta 1644, 107-13.
4. Korsmeyer, S.J. et al. (1993) Semin Cancer Biol 4, 327-32.
5. Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74.
6. Gibson, L. et al. (1996) Oncogene 13, 665-75.
7. O'Reilly, L.A. et al. (2001) Cell Death Differ 8, 486-94.
8. Print, C.G. et al. (1998) Proc Natl Acad Sci U S A 95, 12424-31.
9. Ross, A.J. et al. (1998) Nat Genet 18, 251-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.