MO25α/CAB39 (C49D8) Rabbit mAb

• 产品编号：CST-2716S      品牌：CST       原厂货号：2716S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MO25α/CAB39 (C49D8)兔单抗抗体识别内源性的MO25α/CAB39总蛋白水平。该单克隆抗体用与人类MO25α/CAB39蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。AMPK（AMP激活的蛋白激酶）是一个关键的细胞成分，能够调节能量平衡（1,2）。由于能量耗尽时，AMP/ATP的比率增加，AMPK被在其催化亚基α1和α2磷酸化而激活（3）。研究表明，抑癌基因LKB1在哺乳动物中磷酸化和激活AMPK（4,5）。MO25α（鼠蛋白25α），也称为CAB39（钙结合蛋白39），是体内LKB1复合物的一个组分（5,6）。MO25α/CAB39不直接与LKB1相互作用；相反，它与STRADα有物理性连接（6）。MO25α/CAB39和STRADα共同帮助细胞质中LKB1的锚定（6）。MO25α/CAB39可稳定LKB1-STRADα复合物，并进一步提高LKB1的催化活性（6）。

1. Hardie, D.G. et al. (2003) FEBS Lett 546, 113-20.
2. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Woods, A. et al. (2003) Curr Biol 13, 2004-8.
5. Hawley, S.A. et al. (2003) J Biol 2, 28.
6. Boudeau, J. et al. (2003) EMBO J 22, 5102-14.

原厂资料：

Specificity / Sensitivity

MO25α/CAB39 (C49D8) Rabbit mAb detects endogenous levels of total MO25α/CAB39 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MO25α/CAB39.

Background

AMPK (AMP-activated protein kinase) is a key cellular component that regulates energy homeostasis (1,2). When the AMP/ATP ratio is increased due to energy depletion, AMPK is phosphorylated at its α1 and α2 catalytic subunits and activated (3). Studies showed that the tumor suppressor LKB1 phosphorylates and activates AMPK in mammals (4,5). MO25α (mouse protein 25α), also called CAB39 (calcium binding protein 39), is a component of the LKB1 complex in vivo (5,6). MO25α/CAB39 does not directly interact with LKB1; instead, it is physically associated with STRADα (6). Together MO25α/CAB39 and STRADα help anchor LKB1 in the cytoplasm (6). MO25α/CAB39 stablizes the LKB1-STRADα complex and further increases the catalytic activity of LKB1 (6).

1. Hardie, D.G. et al. (2003) FEBS Lett 546, 113-20.
2. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
4. Woods, A. et al. (2003) Curr Biol 13, 2004-8.
5. Hawley, S.A. et al. (2003) J Biol 2, 28.
6. Boudeau, J. et al. (2003) EMBO J 22, 5102-14.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody