Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody

• 产品编号：CST-2701L      品牌：CST       原厂货号：2701L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody只能检测内源的在Tyr319位点磷酸化的Zap-70蛋白。此抗体与在Tyr352位点磷酸化的Syk蛋白交叉反应。此多克隆抗体是通过合成人源对应的Zap-70 Tyr319位点周围的磷肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析法纯化。Syk 家族酪氨酸激酶Zap-70 在T 细胞和 NK 细胞中表达并在响应T细胞受体(TCR)而介导T细胞激活中发挥了重要的作用(1)。在TCR 招募下, Zap-70 通过自磷酸化和Src家族酪氨酸激酶Lck转磷酸化作用下快速的磷酸化几个酪氨酸残基(2-6)。酪氨酸的磷酸化与Zap-70激酶活性的增加和下游信号传导有关系。Zap-70 的表达与疾病的进程和慢性淋巴白血病患者的生存率有关(7,8)。Tyr319位点的磷酸化对激活PLC-gamma1依赖和Ras依赖在配体引起的T细胞的级联信号反应中形成包含Zap-70信号复合体是必需的(5,6)。Syk的同源基因的Tyr352位点与PLC-gamma1也有关联(9)。

1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
2. Iwashima, M. et al. (1994) Science 263, 1136-9.
3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
9. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Hamster, Monkey, Chicken, Bovine, Dog, Pig, Horse

Specificity / Sensitivity

Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. It cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).Phosphorylation of Tyr319 is required for the assembly of a Zap-70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells (5,6). The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1 (9).

1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
2. Iwashima, M. et al. (1994) Science 263, 1136-9.
3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
9. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.