β-Catenin (L87A12) Mouse mAb

• 产品编号：CST-2698S      品牌：CST       原厂货号：2698S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

β-Catenin (L87A12) Mouse mAb小鼠单抗识别内源性的总β-Catenin 蛋白.此单抗由合成肽段免疫动物产生，合成肽段与β-Catenin的 邻近Asp56氨基酸残基序列一致.β-catenin是Wnt信号通路下游的重要效应分子（1）。在脊椎动物体内它涉及到两个重要的生物过程：早期胚胎发育（2）和肿瘤发生（3）。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化（4-6）。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41，并促使其进一步被降解（7）。这些位点发生突变会提高β-catenin蛋白稳定性，并且已在多种癌细胞株中发现这些突变（8）。

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.

原厂资料：

Specificity / Sensitivity

β-Catenin (L87A12) Mouse mAb detects endogenous levels of total β-catenin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp56 of human β-catenin.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
5. Liu, C. et al. (2002) Cell 108, 837-47.
6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
8. Morin, P.J. et al. (1997) Science 275, 1787-90.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody