AS160 (C69A7) Rabbit mAb

• 产品编号：CST-2670S      品牌：CST       原厂货号：2670S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AS160 (C69A7)兔单克隆抗体识别内源性的AS160总蛋白水平。该单克隆抗体用与人类AS160蛋白中Ala195位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。多肽胰岛素是一种重要的主要激素，控制关键的能量相关功能，如葡萄糖和脂质代谢。胰岛素结合并激活胰岛素受体（IR）酪氨酸激酶，磷酸化并招募不同的接头蛋白。胰岛素及其受体启动的信号转导通路刺激肌肉细胞和脂肪细胞通过从细胞质易位到细胞膜的GLUT4葡萄糖转运体进行葡萄糖摄取（1）。Akt丝氨酸/苏氨酸激酶（AS160，TBC1D4）的一个160 kDa的底物是Rab GTP酶激活的蛋白，它能调节胰岛素刺激的GLUT4运输。AS160在许多组织表达，包括脑，肾，肝，棕色和白色脂肪组织（2）。体内多个AS160的Akt磷酸化位点已经被确定，五个位点（Ser318，Ser570，Ser588，Thr642，Thr751）在胰岛素处理后磷酸化增加（2,3）。 使用重组AS160的研究表明，胰岛素刺激的AS160磷酸化是GLUT4易位的一个关键步骤（3），并且在2型糖尿病患者中减少（4）。14-3-3调节蛋白与Thr642磷酸化的AS160蛋白质相互作用，是GLUT4易位的一个必要步骤（5）。 AMPK对AS160的磷酸化参与调控收缩刺激的GLUT4易位（6）。

1. Watson, R.T. and Pessin, J.E. (2006) Trends Biochem. Sci. 31, 215-222.
2. Kane, S. et al. (2002) J. Biol. Chem. 277, 22115-22118.
3. Sano, H. et al. (2003) J. Biol. Chem. 278, 14599-14602.
4. Karlsson, H.K. et al. (2005) Diabetes 54, 1692-1697.
5. Ramm, G. et al. (2006) J. Biol. Chem. 281, 29174-29180.
6. Kramer, H.F. et al. (2006) J. Biol. Chem. 281, 31478-31485.

原厂资料：

Specificity / Sensitivity

AS160 (C69A7) Rabbit mAb detects endogenous levels of total AS160 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala195 of human AS160.

Background

The polypeptide insulin is a major hormone controlling critical energy functions such as glucose and lipid metabolism. Insulin binds to and activates the insulin receptor (IR) tyrosine kinase, which phosphorylates and recruits different adaptor proteins. The signaling pathway initiated by insulin and its receptor stimulates glucose uptake in muscle cells and adipocytes through translocation of GLUT4 glucose transporter from the cytoplasm to the plasma membrane (1). A 160 kDa substrate of the Akt Ser/Thr kinase (AS160, TBC1D4) is a Rab GTPase-activating protein that regulates insulin-stimulated GLUT4 trafficking. AS160 is expressed in many tissues including brain, kidney, liver, and brown and white fat (2). Multiple Akt phosphorylation sites have been identified on AS160 in vivo, with five sites (Ser318, Ser570, Ser588, Thr642, and Thr751) showing increased phosphorylation following insulin treatment (2,3). Studies using recombinant AS160 demonstrate that insulin-stimulated phosphorylation of AS160 is a crucial step in GLUT4 translocation (3) and is reduced in some patients with type 2 diabetes (4). The interaction of 14-3-3 regulatory proteins with AS160 phosphorylated at Thr642 is a necessary step for GLUT4 translocation (5). Phosphorylation of AS160 by AMPK is involved in the regulation of contraction-stimulated GLUT4 translocation (6).

1. Watson, R.T. and Pessin, J.E. (2006) Trends Biochem. Sci. 31, 215-222.
2. Kane, S. et al. (2002) J. Biol. Chem. 277, 22115-22118.
3. Sano, H. et al. (2003) J. Biol. Chem. 278, 14599-14602.
4. Karlsson, H.K. et al. (2005) Diabetes 54, 1692-1697.
5. Ramm, G. et al. (2006) J. Biol. Chem. 281, 29174-29180.
6. Kramer, H.F. et al. (2006) J. Biol. Chem. 281, 31478-31485.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.