Deltex-2 Antibody

• 产品编号：CST-2663S      品牌：CST       原厂货号：2663S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Deltex-2抗体识别转染水平的总Deltex-2蛋白.多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Deltex-2蛋白Ala607的氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化.Deltex (DTX)编码潜在的E3泛素连接酶，首次在果蝇中被确定，并显示调节Notch信号通路（1）。一些哺乳动物Deltex 同源物已被分离（2,3）。都含有一对WWE重复单元，一个脯氨酸富集区和一个指环结构域（4,5）。Deltex 结合Notch的锚蛋白复制结构域，能在不同系统正向或负向调节Notch信号（4,6）。此外， Deltex能形成同型多聚体（4）。但Deltex显示淋巴发育的Notch信号调控中，这个信号修饰基因可能有一个独立于Notch的转录功能.

1. Xu, T. and Artavanis-Tsakonas, S. (1990) Genetics 126, 665-77.
2. Kishi, N. et al. (2001) Int J Dev Neurosci 19, 21-35.
3. Lehar, S.M. and Bevan, M.J. (2006) Mol Cell Biol 26, 7358-71.
4. Matsuno, K. et al. (1995) Development 121, 2633-44.
5. Zweifel, M.E. et al. (2005) Structure 13, 1599-611.
6. Izon, D.J. et al. (2002) Immunity 16, 231-43.
7. Yun, T.J. and Bevan, M.J. (2003) J Immunol 170, 5834-41.
8. Yamamoto, N. et al. (2001) J Biol Chem 276, 45031-40.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Rat

Specificity / Sensitivity

Deltex-2 Antibody detects transfected levels of total Deltex-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala607 of human Deltex-2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Deltex (DTX) encodes a putative E3 ubiquitin ligase first identified from Drosophila and shown to modulate Notch signaling (1). Several mammalian Deltex homologs have been isolated (2,3), all containing a pair of WWE repeats, a proline-rich region, and a Ring-finger domain (4,5). Deltex binds to the ankyrin-repeat domain of Notch and is capable of regulating Notch signaling positively or negatively in different systems (4,6). In addition, Deltex can also form homo-multimers (4). While Deltex has been implicated in Notch signaling regulation of lymphocyte development, this signal modifier may also have a transcriptional function independent of Notch (3, 6-8).

1. Xu, T. and Artavanis-Tsakonas, S. (1990) Genetics 126, 665-77.
2. Kishi, N. et al. (2001) Int J Dev Neurosci 19, 21-35.
3. Lehar, S.M. and Bevan, M.J. (2006) Mol Cell Biol 26, 7358-71.
4. Matsuno, K. et al. (1995) Development 121, 2633-44.
5. Zweifel, M.E. et al. (2005) Structure 13, 1599-611.
6. Izon, D.J. et al. (2002) Immunity 16, 231-43.
7. Yun, T.J. and Bevan, M.J. (2003) J Immunol 170, 5834-41.
8. Yamamoto, N. et al. (2001) J Biol Chem 276, 45031-40.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.