Stat4 (C46B10) Rabbit mAb

• 产品编号：CST-2653S      品牌：CST       原厂货号：2653S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Stat4 (C46B10) Rabbit mAb 能够检测内源Stat4蛋白总体水平。此单克隆抗体是通过合成对应的Stat4 Lys151位点周围的肽段来免疫动物而获得。Jak-Stat信号通路被很多的细胞因子，生长因子和激素所利用(1)。受体诱导的 Jak家族蛋白的酪氨酸磷酸化引起Stat蛋白的磷酸化导致他们入核，与DNA原件结合并活化转录。Stats所调控反应显著的专一性和范围一部分受控于组织特异性表达不同的细胞因子受体，Jaks 和 Stats同时也与不同的Stat成员偶联到不同的受体上的组合有关（2）。Stat4主要是在脾, 胸腺和睾丸组织中表达，并且作为IL-12的调节蛋白而得到研究(3-8)。

1. Darnell, J.E. et al. (1994) Science 264, 1415-1421.
2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
3. Zhong, Z. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4806-4810.
4. Yamamoto, K. et al. (1994) Mol. Cell Biol. 14, 4342-4349.
5. Jacobson, N.G. et al. (1995) J. Exp. Med. 181, 1755-1762.
6. Bacon, C.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 7307-7311.
7. Thierfelder, W.E. et al. (1996) Nature 382, 171-174.
8. Kaplan, M.H. et al. (1996) Nature 382, 174-177.
9. Visconti, R. et al. (2000) Blood 96, 1844-52.

原厂资料：

Specificity / Sensitivity

Stat4 (C46B10) Rabbit mAb detects endogenous levels of total Stat4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys151 of Stat4.

Background

The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins resulting in their nuclear translocation, binding to specific DNA elements and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8).

1. Darnell, J.E. et al. (1994) Science 264, 1415-1421.
2. Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
3. Zhong, Z. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4806-4810.
4. Yamamoto, K. et al. (1994) Mol. Cell Biol. 14, 4342-4349.
5. Jacobson, N.G. et al. (1995) J. Exp. Med. 181, 1755-1762.
6. Bacon, C.M. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 7307-7311.
7. Thierfelder, W.E. et al. (1996) Nature 382, 171-174.
8. Kaplan, M.H. et al. (1996) Nature 382, 174-177.
9. Visconti, R. et al. (2000) Blood 96, 1844-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide . Store at –20°C. Do not aliquot the antibody.