USP4 Antibody

• 产品编号：CST-2651S      品牌：CST       原厂货号：2651S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

USP4抗体识别内源性的USP4总蛋白。基于序列比对预测该抗体可以与USP4的同源异构体UnpEL和UnpES产生交叉反应。该抗体与USP15无交叉反应。该多克隆抗体经合成与人USP4蛋白His666邻近氨基酸残基序列一致的肽段，免疫动物产生。该抗体由蛋白A和肽亲和层析纯化。泛素化作用酶（UBEs）催化蛋白的泛素化，与去泛素化作用酶(DUB)的过程可逆(1,2)。5种DUB亚家族已经被确定，包括USP, UCH, OTU, MJD和JAMM酶。USP4最初通过Mpv20逆转录病毒插入位点附近小鼠基因的研究确定，最初被认为是泛素核蛋白(UNP)。最初对小鼠cDNA 的分析确定了Usp4/Unp作为与人tre-2/tre-17/USP6原癌基因相关的原癌基因(3,4)。Usp4/Unp随后被发现，含有UBP家族的保守的半胱氨酸和组氨酸盒子(5,6)，并具有DUB活性(7,8)。在早期肺癌组织的研究中，人Usp4, USP4/UNPH的同系物，在小细胞肿瘤和肺腺癌中基因表达升高，表明USP4在肿瘤形成中可能发挥作用(6)。另外一项最近的研究表明几种人癌症中USP4过度表达，并且证实USP4通过负向调节p53水平促进细胞转化(9)。人和鼠USP4被证实与肿瘤抑制蛋白Rb家族相互作用，从而为USP4在细胞转化中发挥机械作用提供了证据(10, 11)。

1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
3. Gupta, K. et al. (1994) Oncogene 9, 1729-31.
4. Gupta, K. et al. (1993) Oncogene 8, 2307-10.
5. Papa, F.R. and Hochstrasser, M. (1993) Nature 366, 313-9.
6. Gray, D.A. et al. (1995) Oncogene 10, 2179-83.
7. Gilchrist, C.A. et al. (1997) J Biol Chem 272, 32280-5.
8. Gilchrist, C.A. and Baker, R.T. (2000) Biochim Biophys Acta 1481, 297-309.
9. Zhang, X. et al. (2011) EMBO J 30, 2177-89.
10. Blanchette, P. et al. (2001) Oncogene 20, 5533-7.
11. DeSalle, L.M. et al. (2001) Oncogene 20, 5538-42.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

USP4 Antibody recognizes endogenous levels of total USP4 protein. Based upon sequence alignment, this antibody is predicted to cross-react with the UnpEL and UnpES isoforms of USP4. This antibody does not cross-react with USP15.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His666 of human USP4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD, and JAMM enzymes. USP4 was originally identified during a survey of murine genes near the Mpv20 retroviral insertion site and intially referred to as Ubiquitous Nuclear Protein (UNP). Analysis of the mouse cDNA originally identified Usp4/Unp as a proto-oncogene related to the human tre-2/tre-17/USP6 proto-oncogene (3,4). Usp4/Unp was subsequently observed to contain the conserved Cys and His boxes of the UBP family (5,6) as well as DUB activity (7,8). In a study of primary lung tumor tissue, it was observed that the human homolog of Usp4, USP4/UNPH, had elevated gene expression levels in small cell tumors and adenocarcinomas of the lung, suggesting a causative role for USP4 in neoplasia (6). Another recent study demonstrated overexpression of USP4 in several types of human cancer and that USP4 positively contributes to cell transformation by negatively regulating p53 levels (9). Both murine and human USP4 have been shown to interact with the Rb family of tumor suppressor proteins, providing additional mechanistic evidence of a role for USP4 in cellular transformation (10, 11).

1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
3. Gupta, K. et al. (1994) Oncogene 9, 1729-31.
4. Gupta, K. et al. (1993) Oncogene 8, 2307-10.
5. Papa, F.R. and Hochstrasser, M. (1993) Nature 366, 313-9.
6. Gray, D.A. et al. (1995) Oncogene 10, 2179-83.
7. Gilchrist, C.A. et al. (1997) J Biol Chem 272, 32280-5.
8. Gilchrist, C.A. and Baker, R.T. (2000) Biochim Biophys Acta 1481, 297-309.
9. Zhang, X. et al. (2011) EMBO J 30, 2177-89.
10. Blanchette, P. et al. (2001) Oncogene 20, 5533-7.
11. DeSalle, L.M. et al. (2001) Oncogene 20, 5538-42.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.