Fibrillarin (C13C3) Rabbit mAb

• 产品编号：CST-2639S      品牌：CST       原厂货号：2639S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Fibrillarin (C13C3) Rabbit mAb兔单抗检测内源性fibrillarin总蛋白。通过人工合成人源fibrillarin蛋白Thr298位点周围相应的多肽片段去免疫动物从而制备出单克隆抗体。Fibrillarin是一种2’-O-甲基转移酶，主要定位于核仁的纤维中心区域和卡哈尔体，即RNA转录和pre-RNA加工区域(1,2)。Fibrillarin与几种结构蛋白、box C/D snoRNA形成复合物，在pre-rRNA加工和甲基化、核糖体组装(ribosome assembly)过程中发挥重要作用。该复合物可以催化rRNA序列特异的2’-O-ribose甲基化(3,4)。Fibrillarin的序列、结构和功能高度保守，Fibrillarin的基因表达对早期胚胎发育有重要作用(5)。

1. Tollervey, D. et al. (1993) Cell 72, 443-57.
2. Wang, H. et al. (2000) EMBO J 19, 317-23.
3. Lafontaine, D.L. and Tollervey, D. (2000) Mol Cell Biol 20, 2650-9.
4. Galardi, S. et al. (2002) Mol Cell Biol 22, 6663-8.
5. Newton, K. et al. (2003) Mol Cell Biol 23, 8519-27.

原厂资料：

Specificity / Sensitivity

Fibrillarin (C13C3) Rabbit mAb detects endogenous levels of total fibrillarin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Thr298 of human fibrillarin.

Background

Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place (1,2). Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence (3,4). The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development (5).

1. Tollervey, D. et al. (1993) Cell 72, 443-57.
2. Wang, H. et al. (2000) EMBO J 19, 317-23.
3. Lafontaine, D.L. and Tollervey, D. (2000) Mol Cell Biol 20, 2650-9.
4. Galardi, S. et al. (2002) Mol Cell Biol 22, 6663-8.
5. Newton, K. et al. (2003) Mol Cell Biol 23, 8519-27.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.