DDX3 Antibody

• 产品编号：CST-2635S      品牌：CST       原厂货号：2635S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DDX3 Antibody能够检测内源性DDX3总蛋白水平。该多克隆抗体是经由合成的人DDX3蛋白近氨基端序列相应的肽段免疫动物而生产的。经过Protein A和亲和层析纯化。RNA 解旋酶的DEAD盒子家族的部分特征是一个共同的D-E-A-D氨基酸基序。这个家族的成员正逐渐壮大，从细菌到哺乳动物的众多生物都存在。DEAD解旋酶在RNA代谢和核糖核蛋白(RNP)加工中具有独特的生物学功能（综述1,2）。DDX3是一个具有多种细胞功能的DEAD盒子家族RNA解旋酶。DDX3是HIV-1病毒转录本的核输出所需要的，很可能是与病毒Rev蛋白和宿主共因子 CRM1形成一个复合物（3）。DDX3是hepatitis C virus (HCV) RNA复制（4）所需要的，且在hepatitis B virus (HBV) 相关的肝细胞肿瘤(HCC)中其表达下调 (5)。最近的研究表明DDX3还可作为一个肿瘤抑制蛋白发挥功能。它的表达可以抑制肿瘤细胞群落形成并提高cdk 抑制因子p21 Waf1/Cip1的表达。在HCC中可见DDX3的低表达（5,6），且在许多鳞状细胞癌症中DDX3出现异常的亚细胞定位（6）。在培养细胞中降低DDX3表达会降低细胞增殖时对血清的依赖并改变cyclin D1和p21 Waf1/Cip1的表达（5）。DDX3的Thr204和Thr323位点可被有丝分裂周期蛋白依赖的激酶cyclin B/cdc2所磷酸化。该磷酸化作用被认为能够导致DDX3功能的丧失以及与之相伴的有丝分裂中核糖体生物合成以及翻译的抑制（7）。

1. Rocak, S. and Linder, P. (2004) Nat Rev Mol Cell Biol 5, 232-41.
2. Linder, P. (2006) Nucleic Acids Res 34, 4168-80.
3. Yedavalli, V.S. et al. (2004) Cell 119, 381-92.
4. Ariumi, Y. et al. (2007) J Virol 81, 13922-6.
5. Chang, P.C. et al. (2006) Oncogene 25, 1991-2003.
6. Chao, C.H. et al. (2006) Cancer Res 66, 6579-88.
7. Sekiguchi, T. et al. (2007) Biochem Biophys Res Commun 356, 668-73.

原厂资料：

Specificity / Sensitivity

DDX3 Antibody detects endogenous levels of total DDX3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human DDX3. Antibodies are purified using protein A and peptide affinity chromatography.

ackground

The DEAD box family of RNA helicases is characterized in part by a common D-E-A-D amino acid motif. The family is composed of a growing number of proteins found in a wide range of organisms from bacteria to mammals. DEAD helicases have distinct biological functions in RNA metabolism and ribonucleoprotein (RNP) processing (reviewed in 1,2). 
 DDX3 is a DEAD box family RNA helicase with diverse cellular functions. DDX3 is required for nuclear export of HIV-1 viral transcripts, possibly in a complex with the viral Rev protein and host cofactor CRM1 (3). DDX3 is required for hepatitis C virus (HCV) RNA replication (4) and its expression is downregulated in hepatitis B virus (HBV) associated hepatocellular carcinoma (HCC) (5). 
 Recent evidence suggests that DDX3 functions as a tumor suppressor protein. Its expression inhibits tumor cell colony formation and increases expression of the cdk inhibitor p21 Waf1/Cip1. Low DDX3 expression has been shown in HCC (5,6), and aberrant subcellular localization occurs in many squamous cell carcinomas (6). Reduced DDX3 expression in cultured cells causes a diminished dependence on serum for cell proliferation and changes in cyclin D1 and p21 Waf1/Cip1 expression (5). 
 DDX3 is phosphorylated at Thr204 and Thr323 by the mitotic cyclin dependent kinase, cyclin B/cdc2. This phosphorylation is thought to cause a loss of DDX3 function and a concomitant repression of ribosome biogenesis and translation in mitosis (7).

1. Rocak, S. and Linder, P. (2004) Nat Rev Mol Cell Biol 5, 232-41.
2. Linder, P. (2006) Nucleic Acids Res 34, 4168-80.
3. Yedavalli, V.S. et al. (2004) Cell 119, 381-92.
4. Ariumi, Y. et al. (2007) J Virol 81, 13922-6.
5. Chang, P.C. et al. (2006) Oncogene 25, 1991-2003.
6. Chao, C.H. et al. (2006) Cancer Res 66, 6579-88.
7. Sekiguchi, T. et al. (2007) Biochem Biophys Res Commun 356, 668-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody