NUP98 (C37G10) Rabbit mAb

• 产品编号：CST-2597S      品牌：CST       原厂货号：2597S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NUP98 (C37G10) Rabbit mAb兔单抗检测内源性NUP98总蛋白。通过人工合成人源NUP98蛋白Leu518位点周围相应的多肽片段去免疫动物从而制备出单克隆抗体。NUP98 (nucleoporin 98 kDa)是核孔复合物的一个成员。首先以三种不同的前体表达，随后这个前体经历自动剪切产生一个共同的氨基端98 kDa大小的多肽(NUP98)和羧基端6, 96 (NUP96)和88 (p88) kDa大小的多肽(1-2)。NUP98蛋白在它的氨基端含有FG和GLFG重复结构域和它的羧基端含有一个RNA结合域(3)。NUP98基因定位在chromosome 11p15.5，该区域在白血病中频繁地重新排列。迄今，对于NUP98蛋白已经确认15个融合伴侣 (4-5)。

1. Fontoura, B.M. et al. (1999) J Cell Biol 144, 1097-112.
2. Rosenblum, J.S. and Blobel, G. (1999) Proc Natl Acad Sci U S A 96, 11370-5.
3. Griffis, E.R. et al. (2004) Mol Biol Cell 15, 1991-2002.
4. Slape, C. and Aplan, P.D. (2004) Leuk Lymphoma 45, 1341-50.
5. Nakamura, T. (2005) Int J Hematol 82, 21-7.

原厂资料：

Specificity / Sensitivity

NUP98 (C37G10) Rabbit mAb detects endogenous levels of total NUP98 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu518 of human NUP98.

Background

NUP98 (nucleoporin 98 kDa) is a component of the nuclear pore complex. It is expressed as three different precursors that undergo auto-cleavage to generate a common amino-terminal 98 kDa peptide (NUP98) and carboxy-terminal 6, 96 (NUP96) and 88 (p88) kDa peptides (1-2). NUP98 contains FG and GLFG repeat domains at its amino terminus and a RNA-binding domain in its carboxy terminus (3). The NUP98 gene is localized on chromosome 11p15.5, a region frequently rearranged in leukemias. To date, 15 fusion partners have been identified for NUP98 (4-5).

1. Fontoura, B.M. et al. (1999) J Cell Biol 144, 1097-112.
2. Rosenblum, J.S. and Blobel, G. (1999) Proc Natl Acad Sci U S A 96, 11370-5.
3. Griffis, E.R. et al. (2004) Mol Biol Cell 15, 1991-2002.
4. Slape, C. and Aplan, P.D. (2004) Leuk Lymphoma 45, 1341-50.
5. Nakamura, T. (2005) Int J Hematol 82, 21-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.