Naked2 (C36D3) Rabbit mAb

• 产品编号：CST-2596S      品牌：CST       原厂货号：2596S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Naked2 (C36D3)Rabbit mAb 兔单抗可以识别被转染的小鼠Naked2蛋白。合成对应小鼠Naked蛋白的Gly47邻近的氨基酸残基序列一致的多肽，免疫动物获得单克隆抗体。果蝇Naked cuticle的同源蛋白Naked1（Nkd1）和Naked2（Nkd2）通过反馈机制负调控Wnt/Wingless信号通路（1,2）。果蝇和脊柱动物Naked蛋白都包括一个可能的钙-结合EF-手区域，但是果蝇Naked并不与钙结合而是与锌结合（3）。Naked通过结合Dishevelled蛋白以控制Dishevelled功能向平面细胞极性途径转化进而抑制经典Wnt/β-catenin信号通路（2,4）。Naked1是Wnt信号通路的直接靶点，在一些结肠癌中过表达，归结于Wnt/β-catenin信号通路的持续激活（5）。豆蔻酰化的Naked2对于促使TGF-α进入极化上皮细胞底外侧质膜是必须的（6）。

1. Zeng, W. et al. (2000) Nature 403, 789-795.
2. Wharton, K.A. et al. (2001) Dev. Biol. 234, 93-106.
3. Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026.
4. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802-3807.
5. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978.
6. Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576.

原厂资料：

Specificity / Sensitivity

Naked2 (C36D3) Rabbit mAb detects transfected levels of mouse Naked2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly47 of mouse Naked2.

Background

Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).

1. Zeng, W. et al. (2000) Nature 403, 789-795.
2. Wharton, K.A. et al. (2001) Dev. Biol. 234, 93-106.
3. Rousset, R. et al. (2002) J. Biol. Chem. 277, 49019-49026.
4. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 3802-3807.
5. Yan, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14973-14978.
6. Li, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 5571-5576.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody