SirT6 Antibody

• 产品编号：CST-2590S      品牌：CST       原厂货号：2590S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SirT6 Antibody能够检测内源性SirT6总蛋白水平。该抗体不能与其它sirtuin蛋白发生交叉反应。通过合成的与人源SirT6蛋白羧基端周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过多肽亲和层析纯化获得。Silent Information Regulator (SIR2)家族基因是高度保守基因组，它们能编码烟酰胺腺呤二核苷酸依赖的蛋白去乙酰化酶，又称为组蛋白去乙酰化酶(histone deacetylase,HDAC3)。这些基因的最初发现和最好的特征是酿酒酵母( Saccharomyces cerevisiae)SIR2，它涉及交配型位点、端粒维持、DNA损伤反应和细胞老化的沉默(1)。SirT3是哺乳动物Sir2的直系同源物，它也是一个细胞核、染色质相关的蛋白，并且促进正常维持基因组完整，其主要通过base excision repair (BER)通路介导(2-4)。BER通路修复单链DNA损伤，而当内源性烷基化、氧化作用和脱氨作用事件时该损伤是自发形成的。SirT6基因敲除的老鼠显示对DNA损伤的试剂敏感度增加，包括烷化剂MMS和H2O2(2)。此外，这些老鼠显示基因组不稳定性，伴随着片段染色体、分离的着丝粒和间距的频繁增加(2)。SirT6蛋白可能通过去乙酰基的DNA Polβ或其它这条通路的中心成分调节BER通路(2)。

1. Guarente, L. (1999) Nat Genet 23, 281-5.
2. Mostoslavsky, R. et al. (2006) Cell 124, 315-29.
3. Liszt, G. et al. (2005) J Biol Chem 280, 21313-20.
4. Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35.

原厂资料：

Specificity / Sensitivity

SirT6 Antibody detects endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins.Source / PurificationPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human SirT6 protein. Antibodies are purified by peptide affinity chromatography.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human SirT6 protein. Antibodies are purified by peptide affinity chromatography.

Background

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H2O2 (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2).

1. Guarente, L. (1999) Nat Genet 23, 281-5.
2. Mostoslavsky, R. et al. (2006) Cell 124, 315-29.
3. Liszt, G. et al. (2005) J Biol Chem 280, 21313-20.
4. Michishita, E. et al. (2005) Mol Biol Cell 16, 4623-35.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.