DLL4 Antibody

• 产品编号：CST-2589S      品牌：CST       原厂货号：2589S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DLL4抗体可以识别内源性的总DLL4蛋白。次抗体由合成的多肽免疫动物获得多克隆抗体，合成的多肽与人DLL4蛋白617位亮氨酸的邻近氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化。Notch受体，单次跨膜I 型蛋白，DSL (Delta, Serrate, Lag2)与配体结合激活Notch信号通路。DSL蛋白包括多个EGF－样重复序列和一个DSL结构域，以结合Notch（1,2）。哺乳动物中已经发现有5个DSL蛋白，包括: Jagged1, Jagged2, Delta-like (DLL) 1, 3和4（3）。配体与Notch受体结合导致受体ADAM蛋白酶和γ-分泌酶复合物经两步溶蛋白性裂解。Notch的细胞内区域经释放转入细胞核内激活转录。Notch配体可能通过类似Notch的方式被处理，提示受体-配体相互作用将导致双向的信号传导（4-6）。DLL4在血管内皮中特异性高表达（7），DLL4单倍剂量不足是导致小鼠血管系统缺陷的主要原因（8-11）。阻遏DLL4可以在动物模型中抑制肿瘤生长（12-14）。

1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
3. Chiba, S. (2006) Stem Cells 24, 2437-2447.
4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.
7. Shutter, J.R. et al. (2000) Genes Dev. 14, 1313-1318.
8. Gale, N.W. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 15949-15954.
9. Krebs, L.T. et al. (2004) Genes Dev. 18, 2469-2473.
10. Duarte, A. et al. (2004) Genes Dev. 18, 2474-2478.
11. Hellström, M. et al. (2007) Nature 445, 776-780.
12. Noguera-Troise, I. et al. (2006) Nature 444, 1032-1037.
13. Lobov, I.B. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3219-3224.
14. Scehnet, J.S. et al. (2007) Blood 109, 4753-4760.

原厂资料：

Specificity / Sensitivity

DLL4 Antibody detects endogenous levels of total DLL4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding residue Leu617 of human DLL4. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6).DLL4 expression is highly restricted to the vascular endothelium (7), and haploinsufficiency of DLL4 results in major defects in vascular systems in mouse (8-11). Blockade of DLL4 inhibits tumor growth in model systems (12-14).

1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868.
2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328.
3. Chiba, S. (2006) Stem Cells 24, 2437-2447.
4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610.
5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643.
6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.
7. Shutter, J.R. et al. (2000) Genes Dev. 14, 1313-1318.
8. Gale, N.W. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 15949-15954.
9. Krebs, L.T. et al. (2004) Genes Dev. 18, 2469-2473.
10. Duarte, A. et al. (2004) Genes Dev. 18, 2474-2478.
11. Hellström, M. et al. (2007) Nature 445, 776-780.
12. Noguera-Troise, I. et al. (2006) Nature 444, 1032-1037.
13. Lobov, I.B. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3219-3224.
14. Scehnet, J.S. et al. (2007) Blood 109, 4753-4760.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody