GLI2 (R770) Antibody

• 产品编号：CST-2585S      品牌：CST       原厂货号：2585S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GLI2 (R770)抗体可以检测转染水平的人GLI2 蛋白。合成与人GLI2 770的邻近氨基酸残基序列一致的多肽，经免疫动物获得多克隆抗体。抗体由肽段亲和层析技术纯化。最初发现GLI是一个在恶性神经胶质瘤中扩大表达的蛋白（1），能够与逆转录病毒E1A一起转化原始细胞（2）。GLI属于锌指蛋白Kruppel家族，哺乳动物有三种GLI蛋白：GLI1, GLI2 和 GLI3 (3).这些GLI蛋白与果蝇同源蛋白Ci（Cubitus interruptus）结构相似，并且作为转录因子被Hedgehog信号通路激活。Hedgehog信号通路在动物发育过程中发挥重要作用，并且在多种癌症中异常激活（4,5）。GLI2包括转录抑制和转录激活区域（6），报道显示GLI2的一些亚型有不同功能（7-9）。在皮肤中过表达GLI2会引起小鼠基底细胞癌（10），而GLI2功能缺失则与脑垂体异常有关（11）。

1. Kinzler, K.W. et al. (1987) Science 236, 70-3.
2. Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
3. Kinzler, K.W. et al. (1988) Nature 332, 371-4.
4. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
5. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
6. Sasaki, H. et al. (1999) Development 126, 3915-24.
7. Tanimura, A. et al. (1998) J Virol 72, 3958-64.
8. Tojo, M. et al. (2003) Br J Dermatol 148, 892-7.
9. Speek, M. et al. (2006) BMC Mol Biol 7, 13.
10. Grachtchouk, M. et al. (2000) Nat Genet 24, 216-7.
11. Roessler, E. et al. (2003) Proc Natl Acad Sci USA 100, 13424-9.

原厂资料：

Specificity / Sensitivity

GLI2 (R770) Antibody detects transfected levels of human GLI2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg770 of human GLI2. Antibodies are purified by peptide affinity chromatography.

Background

GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2 and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Ci (Cubitus interruptus) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development and is aberrantly activated in many types of cancers (4,5).GLI2 contains both transcription repression and activation domains (6) and several isoforms of GLI2 have been reported that may have different activities (7-9). Overexpression of GLI2 in skin causes basal cell carcinoma in mice (10), while loss-of-function of GLI2 is associated with pituitary anomalies (11).

1. Kinzler, K.W. et al. (1987) Science 236, 70-3.
2. Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724-8.
3. Kinzler, K.W. et al. (1988) Nature 332, 371-4.
4. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87.
5. McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114.
6. Sasaki, H. et al. (1999) Development 126, 3915-24.
7. Tanimura, A. et al. (1998) J Virol 72, 3958-64.
8. Tojo, M. et al. (2003) Br J Dermatol 148, 892-7.
9. Speek, M. et al. (2006) BMC Mol Biol 7, 13.
10. Grachtchouk, M. et al. (2000) Nat Genet 24, 216-7.
11. Roessler, E. et al. (2003) Proc Natl Acad Sci USA 100, 13424-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.