Phospho-LRP6 (Ser1490) Antibody

• 产品编号：CST-2568L      品牌：CST       原厂货号：2568L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化LRP6(Ser1490)抗体识别1490位丝氨酸发生磷酸化的内源性LRP6蛋白，另外，预测但仍未确认的是，此抗体也能识别1493位丝氨酸磷酸化时的内源性LRP5，（对应LRP6的丝氨酸1490）多克隆抗体是通过一种合成的磷酸化肽段去免疫动物产生。这种合成的肽段与人源LRP6蛋白邻近1490位丝氨酸的氨基酸残基序列一致，抗体由蛋白A和肽段亲和层析技术纯化 .RP5和LRP6是单次跨膜蛋白，隶属于低密度脂蛋白受体（LDLR）相关蛋白家族。不同于其他LDLR家族成员，LRP5和LRP6胞外域有4个EGF和3个LDLR重复，胞浆域富含脯氨酸（1）。它们在经典Wnt/β-catenin信号通路中作为Wnt的共同受体（2,3）。LRP5和LRP6高度同源并且在发育过程中有多种作用（4,5）。LRP5和LRP6的活性在与Dickkopf (DKK) 家族某些蛋白结合后将被抑制（6,7）。Wnt信号通路被激活后，LRP6可以被如GSK-3和CK1之类的激酶在多个位点被磷酸化，例如1479位苏氨酸，1490位丝氨酸和1493位苏氨酸（8-10）。磷酸化的LRP6招募axin蛋白到膜上以激活β-catenin信号通路（8-10）。

1. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888.
2. Pinson, K.I. et al. (2000) Nature 407, 535-538.
3. Tamai, K. et al. (2000) Nature 407, 530-535.
4. Kelly, O.G. et al. (2004) Development 131, 2803-2815.
5. He, X. et al. (2004) Development 131, 1663-1677.
6. Semënov, M.V. et al. (2001) Curr Biol 11, 951-61.
7. Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668.
8. Tamai, K. et al. (2004) Mol. Cell 13, 149-156.
9. Zeng, X. et al. (2005) Nature 438, 873-877.
10. Davidson, G. et al. (2005) Nature 438, 867-872.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse

Specificity / Sensitivity

Phospho-LRP6 (Ser1490) Antibody detects endogenous levels of LRP6 protein when phosphorylated at serine 1490. It is expected, although not yet confirmed, to also detect endogenous levels of LRP5 protein when phosphorylated at serine 1493 (corresponding to serine 1490 in LRP6).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1490 of human LRP6. Antibodies are purified by protein A and peptide affinity chromatography.

Background

LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10).

1. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248, 879-888.
2. Pinson, K.I. et al. (2000) Nature 407, 535-538.
3. Tamai, K. et al. (2000) Nature 407, 530-535.
4. Kelly, O.G. et al. (2004) Development 131, 2803-2815.
5. He, X. et al. (2004) Development 131, 1663-1677.
6. Semënov, M.V. et al. (2001) Curr Biol 11, 951-61.
7. Bafico, A. et al. (2001) Nat. Cell Biol. 3, 683-668.
8. Tamai, K. et al. (2004) Mol. Cell 13, 149-156.
9. Zeng, X. et al. (2005) Nature 438, 873-877.
10. Davidson, G. et al. (2005) Nature 438, 867-872.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.