RhoGDI Antibody

• 产品编号：CST-2564S      品牌：CST       原厂货号：2564S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RhoGDI Antibody检测内源性RhoGDI总蛋白。通过人工合成人源RhoGDI蛋白相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Rho family small GTPases包括Rho、Rac和cdc42，该家族作为分子开关，调节细胞进程例如细胞迁移、粘附、扩增和分化。它们通过鸟苷酸交换因子（guanine nucleotide exchange factor，GEF)被激活，这将催化已结合的GDP交换成GTP，然而通过GTPase activating proteins (GAPs)被抑制，这将催化GTP的水解成GDP。第三个调节水平通过Rho GDP dissociation inhibitor (RhoGDI)的计量结合力被提供。RhoGDI通过抑制核甘酸交换和细胞膜关联从而影响Rho活性，这调节活性和定位(1, 2)。使用RhoGDI的突变形式已经促使RhoGDI抑制和穿梭的功能分开(3)。GDIs和/或GTPases的磷酸化能调节相互之间的吸附，因此GTPase可调节信号。PAK1激酶使RhoGDI蛋白在serines 101和174位点磷酸化可引起Rac1的释放和激活，但是RhoA不行(4)。

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Dovas, A. and Couchman, J.R. (2005) Biochem J 390, 1-9.
3. Dransart, E. et al. (2005) J Biol Chem 280, 4674-83.
4. DerMardirossian, C. et al. (2004) Mol Cell 15, 117-27.

原厂资料：

Specificity / Sensitivity

RhoGDI Antibody detects endogenous levels of total RhoGDI protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human RhoGDI. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization (Reviewed in 1, 2). The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI (3). Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated signaling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA (4).

1. DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
2. Dovas, A. and Couchman, J.R. (2005) Biochem J 390, 1-9.
3. Dransart, E. et al. (2005) J Biol Chem 280, 4674-83.
4. DerMardirossian, C. et al. (2004) Mol Cell 15, 117-27.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.