Phospho-p57 Kip2 (Thr310) Antibody

• 产品编号：CST-2558S      品牌：CST       原厂货号：2558S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-p57 Kip2 (Thr310) Antibody能够检测内源性苏氨酸（310位）磷酸化的p57 Kip2蛋白。该抗体也许与苏氨酸（187位）磷酸化的p27 Kip1有交叉反应。.该多克隆抗体是由合成的人源的针对p57 Kip2蛋白苏氨酸（310位）的磷酸化肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。p27 Kip1是周期蛋白依赖性激酶抑制剂Cip/Kip家族的一员。同其类似物一样，如p57 Kip2 和p21 Waf1/Cip1，其巩固G1限制点的能力源于CDK2/cyclin E和其它CDK/cyclin复合体的结合抑制。在静止期细胞和cAMP或其它细胞周期阴性调节剂处理的细胞中，p27的表达是上调的。白细胞介素2或者其它有丝分裂原能够诱导p27的下调；该过程涉及p27的磷酸化和泛素化-蛋白酶体途径介导的降解(1-4)。p57 Kip2表达水平通过Skp1/Cul1/F-box-type E3 泛素连接酶复合物 SCF-Skp2被.泛素化/降解途径控制。这一效应依赖于苏氨酸（310位）(5)。p27 Kip1中对应的苏氨酸磷酸化位点为苏氨酸（187位），同样具有调节蛋白稳定性的作用(6)。

1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23.
2. Polyak, K. et al. (1994) Genes Dev 8, 9-22.
3. Kato, J.Y. et al. (1994) Cell 79, 487-96.
4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.
5. Kamura, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 10231-10236.
6. Ishida, N. et al. (2000) J. Biol. Chem. 275, 25146-25154.

原厂资料：

Specificity / Sensitivity

Phospho-p57 Kip2 (Thr310) Antibody detects endogenous levels of p57 Kip2 only when phosphorylated at threonine 310. This antibody may cross-react with p27 Kip1 when phosphorylated at Thr187. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr310 of human p57 Kip2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin 2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4).

Levels of p57 Kip2 are controlled by ubiquitination/degradation via the Skp1/Cul1/F-box-type E3 ubiquitin ligase complex SCF-Skp2, and this effect is dependent on Thr310 (5). A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regulate protein stability (6).

1. Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23.
2. Polyak, K. et al. (1994) Genes Dev 8, 9-22.
3. Kato, J.Y. et al. (1994) Cell 79, 487-96.
4. Vlach, J. et al. (1997) EMBO J 16, 5334-44.
5. Kamura, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 10231-10236.
6. Ishida, N. et al. (2000) J. Biol. Chem. 275, 25146-25154.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody