GFP Antibody

• 产品编号：CST-2555S      品牌：CST       原厂货号：2555S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

GFP Antibody用来检测外源性表达的GFP标签蛋白，请注意GFP标签在融合蛋白分子量上增加了27KDa。多克隆抗体是采用GFP的合成肽段相对应的合成肽段免疫动物生产的，抗体的纯化采用蛋白A和肽亲和色谱法。多克隆抗体是采用GFP的合成肽段相对应的合成肽段免疫动物生产的，抗体的纯化采用蛋白A和肽亲和色谱法。绿色荧光蛋白（GFP）在水母作为生物发光能量转移受体，其最大吸收光波长为395nm，发射谱的最大峰值为509nm。GFP作为一种融合蛋白已经成为报告基因表达，追踪细胞系和蛋白亚细胞定位的重要工具（1）。

1. Miyawaki, A. et al. (2003) Curr. Opin. Chem. Biol. 7, 557-562.

原厂资料：

Specificity / Sensitivity

GFP Antibody detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27 kDa to the molecular weight of the fusion protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic GFP peptide. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1).

1. Miyawaki, A. et al. (2003) Curr. Opin. Chem. Biol. 7, 557-562.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.