CDK2 (78B2) Rabbit mAb

• 产品编号：CST-2546S      品牌：CST       原厂货号：2546S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CDK2 (78B2) Rabbit mAb能够检测内源性CDK2总蛋白，且不与其他CDKs发生交叉反应。该单克隆抗体是由合成的人源的针对CDK2蛋白的肽段免疫动物生产的。周期蛋白依赖性激酶2(p33CDK2)是细胞周期机制的重要组成部分。类似于p34cdc2，激酶活性的调节经由磷酸化状态以及周期蛋白亚基和CDK抑制剂之间的关系。磷酸化抑制通常发生在14位和15位苏氨酸(1)。CDK2-周期蛋白复合体的抑制也与p27 Kip1和 p21 Waf1/Cip1之间的关系有关(2)。CDK2复合体的磷酸化需要cdc25磷酸酶和160位苏氨酸磷酸化引(由CDK7和cyclin H组成的CAK复合体介导)起的14位和15位苏氨酸去磷酸化。 CDK2/周期蛋白E激酶活性对于G1到S期的转换和Rb蛋白的磷酸化具有重要意义。在S期，活化的CDK2/周期蛋白A复合体支配和磷酸化E2F，而活化的CDK2复合体持续在G2期的胞核中存在(5)。

1. Morgan, D.O. (1995) Nature 374, 131-4.
2. Poon, R.Y. et al. (1996) J Biol Chem 271, 13283-91.
3. Gu, Y. et al. (1992) EMBO J 11, 3995-4005.
4. Fesquet, D. et al. (1993) EMBO J 12, 3111-21.
5. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.

原厂资料：

Specificity / Sensitivity

CDK2 (78B2) Rabbit mAb detects endogenous levels of total CDK2 protein. The antibody does not cross-react with other CDKs.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of human CDK2 . 

Background

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5). 

1. Morgan, D.O. (1995) Nature 374, 131-4.
2. Poon, R.Y. et al. (1996) J Biol Chem 271, 13283-91.
3. Gu, Y. et al. (1992) EMBO J 11, 3995-4005.
4. Fesquet, D. et al. (1993) EMBO J 12, 3111-21.
5. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.