Phospho-Paxillin (Tyr118) Antibody

• 产品编号：CST-2541S      品牌：CST       原厂货号：2541S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Paxillin (Tyr118)抗体仅能识别在118位酪氨酸磷酸化后的内源性paxillin蛋白。抗体与其他酪氨酸磷酸化的蛋白没有交叉反应。合成对应人Paxillin蛋白118位酪氨酸及其邻近氨基酸残基序列的多肽免疫动物获得多克隆抗体。抗体经过蛋白A和肽亲和层析纯化。Paxillin是一个多结构域蛋白，位于黏着斑面对细胞外基质的部位（1）。Paxillin是整合素信号一个重要组成部分，Paxillin酪氨酸磷酸化对于整合素介导的细胞骨架重构是必需的（2）。Paxillin由另一个黏着斑组分磷酸化118位酪氨酸，粘着激酶（FAk）（3,4）。118位酪氨酸磷酸化为其他信号分子聚集到黏着斑提供了接头。已有结果显示Crk的SH2结构域能够与Paxillin磷酸化的118位酪氨酸结合（5）。

1. Turner, C.E. (2000) J Cell Sci 113 Pt 23, 4139-40.
2. Burridge, K. et al. (1992) J Cell Biol 119, 893-903.
3. Bellis, S.L. et al. (1995) J Biol Chem 270, 17437-41.
4. Bellis, S.L. et al. (1997) Biochem J 325 ( Pt 2), 375-81.
5. Schaller, M.D. and Parsons, J.T. (1995) Mol Cell Biol 15, 2635-45.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Zebrafish

Specificity / Sensitivity

Phospho-Paxillin (Tyr118) Antibody detects endogenous levels of paxillin only when phosphorylated at tyrosine 118. The antibody does not cross-react with other tyrosine phosphorylated proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Paxillin is a multi-domain protein that localizes primarily to focal adhesion sites to the extracellular matrix (1). Paxillin is one of the key components of integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-Tyr118 may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5).

1. Turner, C.E. (2000) J Cell Sci 113 Pt 23, 4139-40.
2. Burridge, K. et al. (1992) J Cell Biol 119, 893-903.
3. Bellis, S.L. et al. (1995) J Biol Chem 270, 17437-41.
4. Bellis, S.L. et al. (1997) Biochem J 325 ( Pt 2), 375-81.
5. Schaller, M.D. and Parsons, J.T. (1995) Mol Cell Biol 15, 2635-45.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not
aliquot the antibody.