Wnt5a/b (C27E8) Rabbit mAb

• 产品编号：CST-2530P      品牌：Cell Signaling Technology       原厂货号：2530P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Wnt5a/b (C27E8)Rabbit mAb兔单抗识别内源性的Wnt5a和Wnt5b蛋白，另外抗体与一个分子量在55到60kDa左右的蛋白有交叉反应.此单抗由合成肽段免疫动物产生，该肽段与邻近人Wnt5a蛋白异构体1的Glu201和异构体2的 Glu186的氨基酸残基序列一致，此抗体能识别Wnt5a的两种异构体.Wnt家族包括一些分泌糖蛋白，在动物发育中起重要作用（1）。有19个Wnt基因在人的基因组中编码功能特异的Wnt蛋白（2）。Wnt成员结合Frizzled家族7个跨膜蛋白，激活一些信号通路（3）。经典Wnt/ β-catenin通路需要一个来自低密度脂蛋白受体（LDLR）家族的共受体，如LRP5 和LRP6（4）。异常的Wnt通路激活活化与几种癌症相关（5）。Wnt-5a显示通过经典Wnt通路和非经典通路传递信号，并在多种人类肿瘤中上调（6-8）。在黑素瘤中，Wnt5a被认为直接影响细胞迁移和转移（9）。

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
2. Moon, R.T. et al. (1997) Trends Genet. 13, 157-162.
3. Kohn, A.D. and Moon, R.T. (2004) Cell Calcium 38, 439-446.
4. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
5. Giles, R.H. et al. (2003) Biochim. Biophys. Acta. 1653, 1-24.
6. Mikels, A.J. and Nusse, R. (2006) PLoS Biol. 4, e115.
7. Katoh, M. and Katoh, M. (2007) Int. J. Mol. Med. 19, 273-278.
8. Katoh, M. (2005) Oncol. Rep. 14, 1583-1588.
9. Weeraratna, A.T. et al. (2002) Cancer Cell 1, 279-288.

原厂资料：

Specificity / Sensitivity

Wnt5a/b (C27E8) Rabbit mAb detects endogenous levels of Wnt5a and Wnt5b proteins. This antibody cross-reacts with an unidentified protein between 55 and 60 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu201 of Isoform 1 and Glu186 of Isoform 2 of the human Wnt5a protein. This antibody works to detect both isoforms of human Wnt5a protein.

Background

The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins (2). Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family (4). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (5).Wnt-5a has been shown to signal through the canonical Wnt pathways as well as through non-canonical pathways and is up-regulated in various types of human cancers (6-8). In melanoma, Wnt5a is thought to directly affect cell motility and metastasis (9).

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
2. Moon, R.T. et al. (1997) Trends Genet. 13, 157-162.
3. Kohn, A.D. and Moon, R.T. (2004) Cell Calcium 38, 439-446.
4. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
5. Giles, R.H. et al. (2003) Biochim. Biophys. Acta. 1653, 1-24.
6. Mikels, A.J. and Nusse, R. (2006) PLoS Biol. 4, e115.
7. Katoh, M. and Katoh, M. (2007) Int. J. Mol. Med. 19, 273-278.
8. Katoh, M. (2005) Oncol. Rep. 14, 1583-1588.
9. Weeraratna, A.T. et al. (2002) Cancer Cell 1, 279-288.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.