TGF-β Receptor III Antibody

• 产品编号：CST-2519S      品牌：CST       原厂货号：2519S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

III 型TGF-β受体抗体检测内源性的TGF-βIII 型受体蛋白，不与其他家族成员在生理条件下交叉反应。多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与人源III 型TGF-β受体蛋白邻近胞外结构域氨基酸残基一致，抗体由蛋白A和肽段亲和层析技术纯化。转化生长因子β (TGF-β)超家族成员是一类关键的细胞增殖、分化、发育、形态形成和疾病病理机制的调控因子（1-4）。TGF-β通过三种细胞表面受体I型 (RI), II 型(RII) 和III型 (RIII)传递信号。I型 和 II 型受体是丝氨酸/苏氨酸激酶，形成异侧复合物。在配基结合的刺激下，II 型受体与I型受体形成稳定复合物使I型受体激酶磷酸化并激活（5）。III型受体也称β聚糖，是一种跨膜糖蛋白，它有一个大的胞外结构域与TGF-β有高的亲和结合性，但缺乏一个胞质信号结构域（6,7）。III型受体表达能通过递呈配基到信号复合物来调控TGF-β信号。唯一所知的TGF-β直接信号效应器是Smad家族蛋白，这类蛋白从细胞表面直接转导信号至细胞核内来调控靶基因的转录（8,9）。TGF-β III 型受体在骨骼肌分化中上调（10）。

1. Massague, J. et al. (2000) Cell 103, 295-309.
2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
6. López-Casillas, F. et al. (1991) Cell 67, 785-795.
7. Wang, X.F. et al. (1991) Cell 67, 797-805.
8. Derynck, R. et al. (1998) Cell 95, 737-740.
9. Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.
10. Lopez-Casillas, F. et al. (2003) J. Biol. Chem. 278, 382-390.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

TGF-β Receptor III Antibody detects endogenous levels of the type III TGF-β receptor. This antibody does not cross-react with other family members at physiological conditions.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of the type III TGF-β receptor. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).The type III TGF-β receptor is upregulated during skeletal muscle differentiation (10).

1. Massague, J. et al. (2000) Cell 103, 295-309.
2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
6. López-Casillas, F. et al. (1991) Cell 67, 785-795.
7. Wang, X.F. et al. (1991) Cell 67, 797-805.
8. Derynck, R. et al. (1998) Cell 95, 737-740.
9. Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178.
10. Lopez-Casillas, F. et al. (2003) J. Biol. Chem. 278, 382-390.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.